BIO-RAD MCA729F Mouse anti Rat CD147 antibody, clone OX-47,Mouse anti Rat CD147 antibody, clone OX-47

2024-10-22

Mouse anti Rat CD147 antibody, clone OX-47

货号:MCA729F

规格:0.1 mg

价格:2661

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD147

物种:大鼠

宿主:小鼠

抗体亚型:IgG1

克隆号:OX-47

荧光染料:FITC

产品介绍
Mouse anti Rat CD147 antibody, clone OX-47recognizes a membrane glycoprotein of ~42-48 kDa expressed at high levels on activated lymphocytes but lower levels on resting cells (Patersonet al.1987). CD147 is also present on some endothelia (Fossumet al.1991). The OX-47 antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue (Fossumet al.1991).CD147 is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.Mouse anti Rat CD147 antibody, clone OX-47 is suitable for use in Western blotting (Fodaet al.2001).
产品详情
Target SpeciesRat
Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
ImmunogenT blasts from a mixed lymphocyte reaction.
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells from the NS0/1 mouse myeloma cell line.
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Paterson DJet al.(1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.Mol Immunol. 24 (12): 1281-90.2.Fossum, S.et al.(1991) The MRC OX-47 antigen is a member of the immunoglobulin superfamily with an unusual transmembrane sequence.Eur J Immunol. 21 (3): 671-9.3.Cassella, J.P.et al.(1996) Ontogeny of four blood-brain barrier markers: an immunocytochemical comparison of pial and cerebral cortical microvessels.J Anat. 189 ( Pt 2): 407-15.4.Foda, H.D.et al.(2001) Ventilator-induced lung injury upregulates and activates gelatinases and EMMPRIN: attenuation by the synthetic matrix metalloproteinase inhibitor, Prinomastat (AG3340).Am J Respir Cell Mol Biol. 25 (6): 717-24.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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