BIO-RAD MCA596PE Mouse anti Human CD14 antibody, clone UCHM1,Mouse anti Human CD14 antibody, clone UCHM1

2024-10-22

Mouse anti Human CD14 antibody, clone UCHM1

货号:MCA596PE

规格:100 Tests

价格:3893

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD14

物种:人

宿主:小鼠

抗体亚型:IgG2a

克隆号:UCHM1

荧光染料:PE

产品介绍
Mouse anti Human CD14 antibody, clone UCHM1recognizes a cell surface antigen of ~55 kDa, known as CD14.The CD14 molecule is found predominantly on monocytes and macrophages in flow cytometry, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology the CD14 molecule is found to be present on Langerhans cells, follicular dendritic cells, histiocytes and high endothelial venules. Antibodies to the CD14 molecule are known to induce oxidative burst formation. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative (Hogget al.1984). UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
PreparationPurified IgG prepared by affinity chromatography on Protein G
ReconstitutionReconstitute with 1 ml distilled water
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
ImmunogenHuman Thymocytes followed by peripheral blood mononuclear cells.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Hogg, N. & Horton, M.A. (1987) Myeloid antigens: new and previously defined clustersin Leucocyte Typing III White Cell differentiation antigens. Edited by McMichael, A.J.,et al.2.Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.3.Jonker, M.et al. (1989) Reactivity of mAb specific for human CD markers with Rhesus monkey leucocytes.Leucocyte Typing IV. Oxford University Press p 1058-63.4.Hogg, N.et al.(1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.Immunology. 53 (4): 753-67.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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