Bio-Rad MCA1194A647 CD2抗体,Mouse anti Human CD2:Alexa Fluor 647

2024-10-22

Mouse anti Human CD2:Alexa Fluor 647

货号:MCA1194A647,MCA1194A647T

规格:100 Tests/1ml,25 Tests/1ml

价格:3247,1420

产品类型:流式抗体

品牌:Bio-Rad

抗原: CD2

物种:人

宿主:小鼠

抗体亚型:IgG2b

克隆号:LT2

荧光染料:Alexa Fluor 647

产品介绍
Mouse anti Human CD2 antibody, clone LT2recognizes human CD2, a single chain transmembrane glycoprotein of 50 kDa, expressed by T cells, NK cells and thymocytes.CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells.Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.
产品详情

Target Species

Human

Species Cross-ReactivityTarget SpeciesCross Reactivity
Rhesus Monkey
Chimpanzee
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

1% Bovine Serum Albumin

Immunogen

Normal human peripheral blood granulocytes.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Max Ex/Em650/665
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/20
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul of whole blood using the lysed whole blood labelling method.
同型

Description

Product Code

Applications

Pack Size

Mouse IgG2b Negative Control:Alexa Fluor® 647

MCA691A647

F

100 Tests/mL

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Description

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Human Seroblock

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文献
1. Moingeon, P.et al.(1989) The structural biology of CD2.Immunol Rev. 111: 111-44.2. Beyers, A.D.et al.(1989) Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen.Immunol Rev. 111: 59-77.3. Lin, C.W.et al.(2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.Blood. 106 (10): 3567-74.4. Kap, Y.S.et al.(2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.J Histochem Cytochem. 57: 1159-67.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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