| 产品介绍 | | |
| Mouse anti Rat CD86 antibody, clone 24Frecognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages.Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production.Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86 (Maedaet al.1997). |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG conjugated to Alexa Fluor® 647 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Fusion Partners | Spleen cells from immunised Balb/c mice were fused with cells of the P3U1 mouse myeloma cell line. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Immunogen | HTLV-1 transformed Lewis-S1 cells. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®647 | 650 | 665 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Maeda, K.et al.(1997) Characterization of rat CD80 and CD86 by molecular cloning and mAb.Int Immunol. 9 (7): 993-1000.2.Damoiseaux, J.G.et al.(1998) Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells.J Leukoc Biol. 64 (6): 803-9.3.Hanabuchi, S.et al.(2000) Development of human T-cell leukemia virus type 1-transformed tumors in rats following suppression of T-cell immunity by CD80 and CD86 blockade.J Virol. 74: 428-35.4.Kano, M.et al.(1998) A crucial role of host CD80 and CD86 in rat cardiac xenograft rejection in mice.Transplantation. 65: 837-43.5.Tamatani, T.et al.(2000) AILIM/ICOS: a novel lymphocyte adhesion molecule.Int Immunol. 12: 51-5. |
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