BIO-RAD MCA2521T Mouse anti human CD92 antibody, clone VIM-15b,Mouse anti human CD92 antibody, clone VIM-15b

2024-10-22

Mouse anti human CD92 antibody, clone VIM-15b

货号:MCA2521T,MCA2521

规格:25 µg,0.2 mg

价格:935,3043

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD92

物种:人

宿主:小鼠

抗体亚型:IgG2b

克隆号:VIM-15b

荧光染料:Purified

产品介绍
Mouse anti human CD92 antibody, clone VIM-15brecognizes the C-terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70 kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells.CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine.Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10 (Wille, S.et al.). Removal of Sodium Azide is recommended prior to use in functional assays.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
Preparation
  • MCA2521T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  • MCA2521: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenMV4-11 acute monocyte leukaemia cells.
Fusion PartnersSpleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Preservative Stabilisers0.09% Sodium Azide (NaN3)
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/50
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Wille, S.et al.(2001) Characterization of CDw92 as a member of the choline transporter-like protein family regulated specifically on dendritic cells.J Immunol. 167 (10): 5795-804.2.Majdic, O.et al. (1995) Leucocyte Typing V.Oxford University Press, Oxford pp.984-5.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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