BIO-RAD MCA2520F Mouse anti Human CD65s antibody, clone VIM-2,Mouse anti Human CD65s antibody, clone VIM-2-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

BIO-RAD MCA2520F Mouse anti Human CD65s antibody, clone VIM-2,Mouse anti Human CD65s antibody, clone VIM-2

2024-10-22

Mouse anti Human CD65s antibody, clone VIM-2

货号：MCA2520F

规格：0.1 mg

价格：2865

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD65s

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：VIM-2

荧光染料：FITC

产品介绍
Mouse anti Human CD65s antibody, clone VIM-2selectively recognizes the sialylated form of human CD65, known as CD65s (VIM-2 antigen), a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage.CD65s is aberrantly expressed on some acute myeloid leukaemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34.Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (Lund-Johansenet al.1992).
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgM prepared by ammonium sulphate precipitation.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide (NaN3)1%Bovine Serum Albumin
Immunogen	THP1 (human acute monocytic leukaemia cells).
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Gooi, H.C.et al.(1985) Differences in the fine specificities of monoclonal (Class A) antibodies to human myeloid cells.Clin Exp Immunol. 60 (1): 151-8.2.Lund-johansen, F.et al.(1992) Activation of human phagocytes through carbohydrate antigens (CD15, sialyl-CD15, CDw17, and CDw65).J Immunol. 148 (10): 3221-9.3.Knapp, W.et al.(1994) Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis.Cytometry. 18 (4): 187-98.4.Lund-johansen, F.et al.(1990) Flow cytometric assay for the measurement of human bone marrow phenotype, function and cell cycle.Cytometry. 11 (5): 610-6.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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BIO-RAD MCA2520F Mouse anti Human CD65s antibody, clone VIM-2,Mouse anti Human CD65s antibody, clone VIM-2

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