Bio-Rad MCA2359Z mouse anti PIG TNF alpha:Preservative Free,mouse anti PIG TNF alpha:Preservative Free

2024-10-22

mouse anti PIG TNF alpha:Preservative Free

货号:MCA2359Z

规格:0.5 mg

价格:7931

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:TNF Alpha

物种:pig

宿主:小鼠

抗体亚型:IgG1

克隆号:4F4

荧光染料:Preservative Free

产品介绍
Mouse anti Porcine TNF alpha antibody, clone 6E10recognizes porcine tumour necrosis factor alpha (TNF alpha). Mouse anti Porcine TNF alpha antibody, clone 6E10 recognizes both native and recombinant TNF alpha.TNF alpha is expressed by a range of cells including macrophages and monocytes, and is an important mediator of the porcine inflammatory response.Mouse anti Porcine TNF alpha antibody, clone 6E10 is reported to neutralize the effects of porcine TNF alpha inin vitrocytotoxicity assays using WEHI 164 clone 13 murine fibrosarcoma cells (Suet al.1992).
产品详情
Target SpeciesPig
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenRecombinant porcine TNF alpha.
Approx. Protein ConcentrationsIgG concentration 0.5 mg/ml
Preservative StabilisersNone present
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Store at -20℃.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Su, X.et al. (1992) Monoclonal antibodies to porcine tumor necrosis factor alpha development of an enzyme-linked immunoabsorbant assay.J. Immunoassay 13:253-272.2.Zelnickova, P.et al.(2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.J Immunol Methods. 327: 18-29.3.Zelnickova, P.et al.(2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.Vet Immunol Immunopathol. 124: 367-78.

技术参数

产品应用 ELISA;FN

产品类型 单克隆抗体

推荐用于 ELISA;FN

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