BIO-RAD MCA2041C Mouse anti Bovine CD172a antibody, clone CC149,Mouse anti Bovine CD172a antibody, clone CC149

2024-10-22

Mouse anti Bovine CD172a antibody, clone CC149

货号:MCA2041C

规格:100 Tests/1ml

价格:4208

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD172a

物种:Bovine

宿主:小鼠

抗体亚型:IgG2b

克隆号:CC149

荧光染料:PE-Cy5

产品介绍
Mouse anti Bovine CD172a antibody, clone CC149recognizes bovine CD172a, also known as MyD-1 antigen and SIRPA.CD172a is a ~55 kDa single pass type 1 membrane protein belonging to the family of signal regulatory proteins (SIRP). CD172a has been identified as the receptor for CD47. Bovine CD172a is strongly expressed by splenic macrophages, monocytes and a subset of afferent lymph veiled cells (ALVC) and by dendritic cells in the skin.
产品详情
Target SpeciesBovine
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) -Cy5 - lyophilized
ReconstitutionReconstitute with 1.0ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenBovine afferent veiled dendritic cells
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE-Cy5 488nm laser596667
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Howard, C.J.et al.(1999) Dendritic cells in cattle: phenotype and function.Vet Immunol Immunopathol. 72 (1-2): 119-24.2.Price, S.J. & Hope, J.C.. (2009) Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture withMycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals.Immunology. 126:201-83.Waters, W.R. (2009) Signal regulatory protein alpha (SIRPalpha) cells in the adaptive response to ESAT-6/CFP-10 protein of tuberculous mycobacteria.PLoS One. 4: e6414.4.Brackenbury, L.S.et al.(2005) Identification of a cell population that produces alpha/beta interferonin vitroandin vivoin response to noncytopathic bovine viral diarrhea virus.J Virol. 79: 7738-44.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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