产品介绍 | | |
Mouse anti Human CD161 antibody, clone B199.2recognizes the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A. CD161 is a 225 amino acid ~25 kDa predicted molecular mass, single pass type II transmembrane glycoprotein with a singleC-type lectindomain. CD161 is expressed by almost all NK cells and a subset of CD3+ve T cells (Lanier 1994).CD161, a member of the C-lectin is expressed as a disulphide bond-linked homodimeric cell surface protein, comprising two chains of ~40-44 kDa (Lanieret al.1994). CD161 acts as a receptor for another c-type lectin, LLT1 with roles in the regulation of NK cell and T cell function (Aldemiret al.2005).Mouse anti Human CD161 antibody, clone B199.2 cross-competes with and recognizes a similar epitope to the DX1 monoclonal antibody (Lanieret al.1994). |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Alexa Fluor 647 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
Immunogen | Purified human NK cells cultured in IL-2 (Bennettet al.1996) |
Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
Alexa Fluor®647 | 650 | 665 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
存储条件 | | |
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | Neat |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
|
数据 |
Figure A. PE-A750 conjugated mouse anti human CD3 (MCA463P750) and A647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. PE-750 conjugated mouse anti human CD3 (MCA463P750) and A647 conjugated mouse anti human CD161 (MCA1855A647). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5™ cell analyzer. |
同型 |
Description | Product Code | Applications | Pack Size |
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928AF647 | Flow | 100 Tests |
相关产品 |
Description | Product Code | Applications | Pack Size |
Mouse Seroblock FcR | BUF041A | Flow | 0.1 mg |
Mouse Seroblock FcR | BUF041B | Flow | 0.5 mg |
文献 |
1. Bennett, I.M.et al.(1996) Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiatesin vitroin the presence of interleukin 12.J Exp Med. 184 (5): 1845-56.2. Azzoni, L.et al.(1998) Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells.J Immunol. 161 (7): 3493-500.3. de Lalla, C.et al.(2011) Invariant NKT Cell Reconstitution in Pediatric Leukemia Patients Given HLA-Haploidentical Stem Cell Transplantation Defines Distinct CD4+ and CD4- Subset Dynamics and Correlates with Remission State.J Immunol. 186: 4490-9.4. Huarte, E.et al.(2008) PILAR is a novel modulator of human T-cell expansion.Blood. 112: 1259-68.5. Williams, P.J.et al.(2009) Altered decidual leucocyte populations in the placental bed in pre-eclampsia and foetal growth restriction: a comparison with late normal pregnancy.Reproduction. 138: 177-84.6. Higai, K.et al.(2006) Binding of sialyl Lewis X antigen to lectin-like receptors on NK cells induces cytotoxicity and tyrosine phosphorylation of a 17-kDa protein.Biochim Biophys Acta. 1760 (9): 1355-63.7. Birchall, M.A.et al.(2008) Immunologic response of the laryngeal mucosa to extraesophageal reflux.Ann Otol Rhinol Laryngol. 117: 891-5.8. Bossard, C.et al.(2012) Plasmacytoid dendritic cells and Th17 immune response contribution in gastrointestinal acute graft-versus-host disease.Leukemia. 26: 1471-4.9. Richter, J.et al.(2010) CD161 receptor participates in both impairing NK cell cytotoxicity and the response to glycans and vimentin in patients with rheumatoid arthritis.Clin Immunol. 136: 139-47.10. Abrahamsson, S.V.et al.(2013) Non-myeloablative autologous haematopoietic stem cell transplantation expands regulatory cells and depletes IL-17 producing mucosal-associated invariant T cells in multiple sclerosis.Brain. 136: 2888-903.11. Rother, S.et al.(2015) The c.503T>C Polymorphism in the Human KLRB1 Gene Alters Ligand Binding and Inhibitory Potential of CD161 Molecules.PLoS One. 10 (8): e0135682. |