Bio-Rad MCA1853T CD163抗体,mouse anti human CD163-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1853T CD163抗体,mouse anti human CD163

2024-10-22

mouse anti human CD163

货号：MCA1853T ,MCA1853

规格：25 µg,0.2 mg

价格：893,3723

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD163

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：EDHu-1

荧光染料：Purified

产品介绍
Mouse anti Human CD163 antibody, clone EDHu-1recognizes the human CD163 cell surface antigen, a 130-140 kDa glycoprotein expressed by tissue macrophages. CD163 expression may be induced on monocytes by culture in dexamethasone.Clone EDHu-1 is reported to inhibit the binding of haptoglobin/hemoglobin to CD163 (Madsenet al.2004).Truncation mutation analysis demonstrates binding of EDHu-1 occurs via the N-terminal region of CD163 containing the first three scavenger receptor, Cysteine-rich,SRCR domainsthe third domain being critical as, cleavage of this domain at the major cleavage siteASP-265abrogates binding to the N-terminal fragment.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Rhesus Monkey	√
Sheep	√
Pig	√
Guinea Pig	√
Bovine	√
Cynomolgus monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Carrier Free	Yes
Immunogen	Leucocytes harvested from the pleural cavity of patients with idiopathic spontaneous pneumothorax
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
Flow Cytometry	√	1/10	1/50
Immunoassay	√
Immunofluorescence	√
Immunohistology - Frozen	√
Immunohistology - Paraffin	√
Functional Assays	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
数据
Detection of Human CD163 with Mouse anti Human CD163 (MCA1853) in a Western blot analysis of Human lung tissue lysate with Goat anti Mouse IgG:HRP (STAR207P) at a 1/10000 dilution. Different dilutions of MCA1853 are shown on lanes 2,4, 6 and 8 at 1/200, 1/500, 1/1000 and 1/2000, respectively. Human lung lysate probed with STAR207P only at a 1/10000 dilution was included as a secondary only control (Lane 10)
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 Tests
相关产品
Description	Product Code	Applications	Pack Size
Mouse Seroblock FcR	BUF041A	Flow	0.1 mg
Mouse Seroblock FcR	BUF041B	Flow	0.5 mg
文献
1. Frafjord, A.et al.(2020) Antibody combinations for optimized staining of macrophages in human lung tumours.Scand J Immunol. 92 (1): e12889.2. Ma, H.et al.(2021) Structural comparison of CD163 SRCR5 from different species sheds some light on its involvement in porcine reproductive and respiratory syndromevirus-2 infectionin vitro..Vet Res. 52 (1): 97.3. Kemmerer, C.L.et al.(2021) Cerebrospinal fluid cytokine levels are associated with macrophage infiltration into tumor tissues of glioma patientsBMC Cancer. 21(1):1108.4. Lehmann, M.et al.(2021) New onset of mainly guttate psoriasis after COVID-19 vaccination: a case report.J Eur Acad Dermatol Venereol. 35 (11): e752-e755.5. Baumann, D.et al.(2021) p38 MAPK signaling in M1 macrophages results in selective elimination of M2 macrophages by MEK inhibition.J Immunother Cancer.9 (7): e002319.6. Ikezumi, Y.et al.(2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.Pediatr Nephrol. 36 (2): 349-59.7. Eligini, S.et al.(2019) Biological profile of monocyte-derived macrophages in coronary heart disease patients: implications for plaque morphology.Sci Rep. 9 (1): 8680.8. Edwards, J.H.et al.(2021) Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.Biomaterials. 279: 121204.9. Ströbel, S.et al.(2021) A 3D primary human cell-based in vitro model of non-alcoholic steatohepatitis for efficacy testing of clinical drug candidates.Sci Rep. 11 (1): 22765.10. Galea, I.et al.(2022) Iron Deposition in the Brain After Aneurysmal Subarachnoid Hemorrhage.Stroke. 53 (5): 1633-42.

技术参数

产品应用 IHC(F); ELISA; Flow； IF； IY ；IHC(P); WB

产品类型 Monoclonal Antibody

推荐用于 IHC(F); ELISA; Flow； IF； IY ；IHC(P); WB

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-40065.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

Bio-Rad MCA1853T CD163抗体,mouse anti human CD163

相关推荐