| 产品介绍 | | |
| Mouse anti Human CD81 antibody, clone 1D6recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular leucocytes including activation, proliferation and differentiation.Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects. |
| 产品详情 | | |
Target Species | Human |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Chimpanzee | √ |
| Sheep | √ |
| Goat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Alexa Fluor 488 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide(NaN3) 1% Bovine Serum Albumin |
| Immunogen | OCI-LY8 cells aggregated by 5A6 (another CD81 antibody) |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Fusion Partners | Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | Flow | 100 Tests/1ml |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse Seroblock FcR | BUF041A | Flow | 0.1 mg |
| Mouse Seroblock FcR | BUF041B | Flow | 0.5 mg |
| 数据 |
| Figure A. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Alexa Fluor 488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. RPE conjugated Mouse anti Human CD19 (MCA1940PE) and Alexa Fluor 488 conjugated Mouse anti Human CD81 (MCA1847A488). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5™ cell analyzer. |
| 文献 |
| 1. Schick, M.R. & Levy, S. (1993) The TAPA-1 molecule is associated on the surface of B cells with HLA-DR molecules.J Immunol. 151 (8): 4090-7.2. Levy, S.et al.(1998) CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system.Annu Rev Immunol. 16: 89-109.3. Griebel, P.J.et al.(2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.Vet Immunol Immunopathol. 119: 115-22.4. Welton, J.L.et al(2010) Proteomics analysis of bladder cancer exosomes.Mol Cell Proteomics. 9: 1324-38.5. Davis, W.C.et al.(2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.Vet Immunol Immunopathol. 119: 123-30.6. Flint, M.et al.(1999) Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.J Virol. 73:6235-44.7. Parthasarathy, V.et al.(2009) Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.Immunology. 127: 237-48.8. Rohlena, J.et al.(2009) Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion.Cardiovasc Res. 81: 187-96.9. Ventress, J.K.et al.(2016) Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes.PLoS One. 11 (7): e0160387.10. Mleczko, J.et al.(2018) Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.Mol Nutr Food Res. 62 (5)Feb 20 [Epub ahead of print]. |
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