Bio-Rad MCA1841 CD117抗体,mouse anti human CD117

2024-10-22

mouse anti human CD117

货号:MCA1841

规格:0.1 mg

价格:3400

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD117

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:104D2

荧光染料:Purified

产品介绍
Mouse anti Human CD117 antibody, clone 104D2recognizes the human CD117 cell surface antigen, a 145 kDa glycoprotein also known as c-kit. CD117 is expressed by some haematopoietic stem cells (approx. 70% of CD34+ve cells in normal bone marrow are CD117+ve), some acute myeloid leukaemias and by mast cells.Functionally CD117 is a receptor for stem cell factor, and has receptor tyrosine kinase activity.Mouse anti Human CD117 antibody, clone 104D2 is non-neutralizing.
产品详情

Target Species

Mouse

Species Cross-ReactivityTarget SpeciesCross Reactivity
Cynomolgus monkey
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

ImmunogenMOLM-1 megakaryocyte cell line
Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Regulatory

For research purposes only

Guarantee

Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

1/21/20
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
同型
DescriptionProduct CodeApplicationsPack Size

Mouse IgG1 Negative Control

MCA928

F

100 Tests

文献
1. Broudy, V.C.et al.(1998) Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer.Blood. 91 (3): 898-906.2. Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.3. Jayapal, M.et al.(2006) Genome-wide gene expression profiling of human mast cells stimulated by IgE or FcepsilonRI-aggregation reveals a complex network of genes involved in inflammatory responses.BMC Genomics. 7: 210.4. Randall, V.A.et al.(2008) Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles.J Endocrinol. 197: 11-23.5. Venkata, N.S.G.et al.(2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.PLoS One. 13 (2): e0192244.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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