Bio-Rad MCA1828GA GM-CSF抗体,mouse anti bovine GM-CSF-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1828GA GM-CSF抗体,mouse anti bovine GM-CSF

2024-10-22

mouse anti bovine GM-CSF

货号：MCA1828GA

规格：0.1 mg

价格：2185

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：GM-CSF

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：CC305

荧光染料：Purified

产品介绍
Mouse anti Bovine GM-CSF antibody, clone CC305recognizes bovine granulocyte macrophage colony-stimulating factor (GM-CSF) also known as CSF-2. Bovine GM-CSF is a 143 amino acid cytokine which stimulates proliferation and differentiation of hematopoietic precursors.Mouse anti Bovine GM-CSF antibody, clone CC305 has been used as a capture antibody in conjunction with Mouse anti Sheep GM-CSF, clone 3C2 as a detection reagent in a sandwich ELISA for the measurement of bovine GM-CSF produced by peripheral blood mononuclear cellsin vitro(Kennedyet al.2002).
产品详情
Target Species	Bovine
Species Cross-Reactivity	Target Species	Cross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Carrier Free	Yes
Immunogen	T-end.1, a pMT transformed endothelial cell line.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised C57/BL mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√		5 ug/ml as coating antibody
Western Blotting
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. ELISAThis product may be used as a capture antibody in a sandwich ELISA together withMCA1924when conjugated to biotin as the detection reagent.
数据
Bovine GM-CSF ELISARecombinant Bovine GM-CSF detected using Mouse anti Bovine GM-CSF (MCA1828GA) as the capture reagent and a bovine reactive Mouse anti Sheep GM-CSF, clone 3C2 (MCA1924) conjugated to Biotin as the detection reagent followed by Streptavidin:HRP
文献
1. Kennedy HEet al.(2002) Modulation of immune responses toMycobacterium bovisin cattle depleted of WC1(+) gamma delta T cells.Infect Immun. 70 (3): 1488-500.2. Boutet, P.et al.(2004) Delayed neutrophil apoptosis in bovine subclinical mastitis.J Dairy Sci. 87 (12): 4104-14.3. Bao, Z.J.et al.(2014) Recombinant bovine interferon-τ enhances in vitro development of bovine embryos by upregulating expression of connexin 43 and E-cadherin.J Dairy Sci. 97 (11): 6917-25.4. Pedersen, L.G.et al.(2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal speciesVet Immunol Immunopathol. 88: 111-22.5. Davies, C.J.et al.(2004) Methods of minimizing immunological rejection of a nuclear transfer fetus.U.S. Patent Application: US 2004/0029825 A1

技术参数

产品应用 ELISA;WB

产品类型 Monoclonal Antibody

推荐用于 ELISA;WB

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Bio-Rad MCA1828GA GM-CSF抗体,mouse anti bovine GM-CSF

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