BIO-RAD MCA1615PE Mouse anti Human CD54:RPE,Mouse anti Human CD54:RPE

2024-10-22

Mouse anti Human CD54:RPE

货号:MCA1615PE

规格:100 Tests

价格:3893

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:CD54

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

荧光染料:PE

产品介绍
Mouse anti Human CD54 antibody, clone 15.2recognizes the human CD54 cell surface antigen also known as intracellular Adhesion Molecule -1 (ICAM-1) or Major group rhinovirus receptor.CD54 is expressed by many cells following activation by inflammatory mediators. It is a 505 amino acid with an additional 27 amino acid signal peptide ~90 kDa single pass type I transmembrane glycoprotein bearing 5 Ig-like C2-type domains.Mouse anti Human CD54 antibody, clone 15.2 blocks CD54 function (Berendtet al.1992). Mouse anti Human CD54 antibody, clone 15.2 binds to an epitope on the N-terminal Ig-like domain within a region designated the L43 loop (Chakravorty and Craig 2005).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
ReconstitutionReconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer SolutionPhosphate buffered saline
ImmunogenHuman monocytes
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Pacific Blue®496578
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0-Ag14 myeloma cell line
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light.Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
同型对照
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:RPEMCA928PEF100 Tests/1ml
文献
1.Hadam, M.R. (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.Edited by Knapp, W.et al. Oxford University Press pp 694-697.2.Holmes, C.H.et al.(1990) Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy.J Immunol. 144 (8): 3099-105.3.O'Brien, D.P.et al.(2009) Regulation of the Helicobacter pylori cellular receptor decay-accelerating factor.J Biol Chem. 283: 23922-30.4.Yang, P.et al.(2009) Expression and modulation of RPE cell membrane complement regulatory proteins.Invest Ophthalmol Vis Sci. 50: 3473-81.

技术参数

产品应用 Flow

产品类型 单克隆抗体

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