Bio-Rad MCA1709T MDM2 抗体,Mouse anti MDM2 antibody, clone SMP14-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1709T MDM2 抗体,Mouse anti MDM2 antibody, clone SMP14

2024-10-22

Mouse anti MDM2 antibody, clone SMP14

货号：MCA1709T,MCA1709

规格：20 µg,0.1 mg

价格：1037,2576

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：MDM2

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1

克隆号：SMP14

荧光染料：Purified

产品介绍
Mouse anti MDM2 antibody, clone SMP14recognizes the ~90 kDa MDM2 protein, which forms a complex with p53 leading to p53 inhibition.MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI.MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Mouse	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	MCA1709: Purified IgG prepared by affinity chromatography on Protein A MCA1709T: Purified IgG prepared by affinity chromatography on Protein G
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin¹	√	1/200	1/500
Immunohistology - Resin	√
Immunohistology - Frozen	√
Immunoprecipitation	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. EDTA pH8.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Histology Positive Control TissueBreast Carcinoma
数据
Staining of human breast carcinoma. Formalin fixed, paraffin processed tissue with Mouse anti Human MDM2 (MCA1709T).
文献
1.Picksley, S.M.et al.(1994) Immunochemical analysis of the interaction of p53 with MDM2;--fine mapping of the MDM2 binding site on p53 using synthetic peptides.Oncogene. 9 (9): 2523-9.2. Martin, K.et al.(1995) Stimulation of E2F1/DP1 transcriptional activity by MDM2 oncoprotein.Nature. 375 (6533): 691-4.3. Gueven, N.et al.(2006) Defective p53 response and apoptosis associated with an ataxia-telangiectasia-like phenotype.Cancer Res. 66: 2907-12.

技术参数

产品应用 Immunohistology - Frozen;Immunohistology - Paraffin ;Immunohistology - Resin;Immunoprecipitation

产品类型 Monoclonal Antibody

推荐用于 Immunohistology - Frozen;Immunohistology - Paraffin ;Immunohistology - Resin;Immunoprecipitation

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Bio-Rad MCA1709T MDM2 抗体,Mouse anti MDM2 antibody, clone SMP14

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