Bio-Rad MCA1704 p53抗体,Mouse anti Human p53 antibody, clone DO-11-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1704 p53抗体,Mouse anti Human p53 antibody, clone DO-11

2024-10-22

Mouse anti Human p53 antibody, clone DO-11

货号：MCA1704

规格：0.1 mg

价格：2576

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：p53

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号： DO-11

荧光染料：Purified

产品介绍
Mouse anti Human p53 antibody, clone DO-11recognizes the human Cellular tumor antigen p53, also known as p53 tumor suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637).DO-11 binds to an epitope within the central region of p53 between amino acids181 - 190defining a cryptic epitope exposed in unfolded/ denatured p53 (Shimitzuet al.2006). Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. Mouse anti Human p53 antibody, clone DO-11 recognizes mutant forms of p53 (Warnocket al.2011) and has been used successfully for detection of p53 by western blotting (Weiet al.2012).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	Recombinant human p53.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin¹	√		1/1000
Immunohistology - Frozen	√
Immunoprecipitation	√
Western Blotting	√	1/200	1/2000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Histology Positive Control TissueColon or breast carcinoma
数据
Western-Blot analysis of HEK293 whole cell lysate probed with Mouse anti Human p53 (aa181-190)MCA1704used at 1/200, 1/500, 1/1,000 and 1/2,000 and followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000STAR207P) and visualized on the ChemiDoc MP with 17.5 second exposure.
文献
1. Vojtesek, B.et al.(1995) Conformational changes in p53 analysed using new antibodies to the core DNA binding domain of the protein.Oncogene. 10 (2): 389-93.2. Palecek, E.et al.(2001) Binding of p53 and its core domain to supercoiled DNA.Eur J Biochem. 268: 573-81.3. Coomber, D.W. and Ward, .R.L. (2001) Isolation of human antibodies against the central DNA binding domain of p53 from an individual with colorectal cancer using antibody phage display.Clin Cancer Res. 7: 2802-8.4. Wei, J.et al.(2012) Pathogenic bacterium Helicobacter pylori alters the expression profile of p53 protein isoforms and p53 response to cellular stresses.Proc Natl Acad Sci U S A. 109: E2543-50.5. Warnock, L.J.et al.(2011) Aurora A mediates cross-talk between N- and C-terminal post-translational modifications of p53.Cancer Biol Ther. 12: 1059-68.

技术参数

产品应用 IHC;IP;WB

产品类型 Monoclonal Antibody

推荐用于 IHC;IP;WB

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Bio-Rad MCA1704 p53抗体,Mouse anti Human p53 antibody, clone DO-11

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