Bio-Rad MCA1659 IL-6,Mouse anti Sheep Interleukin-6 antibody, clone 4B6

2024-10-22

Mouse anti Sheep Interleukin-6 antibody, clone 4B6

货号:MCA1659

规格:0.25 mg

价格:5670

产品类型:流式抗体

品牌:Bio-Rad

抗原:IL-6

物种:绵羊

宿主:小鼠

抗体亚型:IgG1

克隆号:4B6

荧光染料:Purified

产品介绍
Mouse anti Sheep Interleukin-6 antibody, clone 4B6recognizes ovine interleukin-6 (IL-6) and has also been reported to recognise recombinant human IL-6 and bovine IL-6 transfected cells. Mouse anti Sheep Interleukin-6 antibody, clone 4B6 does not cross react with ovine IL-1 beta, IL-8, MCP-1 or TNF alpha.
产品详情

Target Species

Sheep

Species Cross-ReactivityTarget SpeciesCross Reactivity
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN3)

ImmunogenRecombinant ovine IL-6.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry 1
ELISA5ug/ml (as a coating antibody)
Western Blotting
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 1x106cells in 100ul.
  • ELISAMCA1659 may also be used in combination withAHP424in a sandwich ELISA assays for ovine IL-6.
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative ControlMCA928Flow100 Tests
文献

1. Herry, C.L.et al.(2016) Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.PLoS One. 11 (4): e0153515.2. Ciliberti, M.G.et al.(2017) Peripheral blood mononuclear cell proliferation and cytokine production in sheep as affected by cortisol level and duration of stress.J Dairy Sci. 100 (1): 750-756.3. Westover, A.et al.(2017) Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep.Front Physiol. 8: 871.4. Castel, A.et al.(2021) Recording and manipulation of vagus nerve electrical activity in chronically instrumented unanesthetized near term fetal sheep.J Neurosci Methods. 360: 109257.5. Ciliberti, M.G.et al.(2022) Green extraction of bioactive compounds from wine lees and their bio-responses on immune modulation usingin vitro.sheep model.J Dairy Sci. Mar 17 [Epub ahead of print].6. Cao, M.et al. (2022) The vagus nerve regulates immunometabolic homeostasis in the ovine fetus near term: impact on terminal ileumasXiv 2203.149677. Damiano, S.et al.(2022) Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambsIt J anim Sci 21 (1): 934-42.

技术参数

产品应用 ELISA;WB;Flow

产品类型 Monoclonal Antibody

推荐用于 ELISA;WB;Flow

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