Bio-Rad MCA1360D405GA V5-Tag抗体,mouse anti V5-TAG:Dylight®405-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1360D405GA V5-Tag抗体,mouse anti V5-TAG:Dylight®405

2024-10-22

mouse anti V5-TAG:Dylight®405

货号：MCA1360D405GA

规格：0.1 mg

价格：1377

产品类型：流式抗体

品牌：Bio-Rad

抗原：V5-Tag

物种：Virus

宿主：小鼠

抗体亚型：IgG2a

克隆号：SV5-Pk1

荧光染料：DyLight 405

产品介绍
Mouse anti V5-Tag, clone SV5-Pk1recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunnet al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randallet al.1993andZhaoet al.2005).
产品详情
Target Species	Viral
Product Form	Purified IgG conjugated to DyLight®405 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Immunogen	Paramyxovirus Simian-Virus 5 (SV5)
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Dylight®405	400	420
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
Acknowledgements	DyLight®is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/100	1/1000
Immunofluorescence	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
文献
1. Kovács, H.A.et al. (2021) Characterization of the Proprotein Convertase-Mediated Processing of Peroxidasin and Peroxidasin-like ProteinAntioxidants. 10 (10): 1565.2. Lucas, R.M.et al.(2021) SCIMP is a spatiotemporal transmembrane scaffold for Erk1/2 to direct pro-inflammatory signaling in TLR-activated macrophages.Cell Rep. 36 (10): 109662.3. Kao, C.Y.et al.(2021) Evidence for complex dynamics during U2 snRNP selection of the intron branchpoint.Nucleic Acids Res. 49 (17): 9965-97.4. Forey, R.et al.(2021) A Role for the Mre11-Rad50-Xrs2 Complex in Gene Expression and Chromosome Organization.Mol Cell. 81 (1): 183-197.e6.4. Hassler, M.et al.(2019) Structural Basis of an Asymmetric Condensin ATPase Cycle.Mol Cell. 74 (6): 1175-1188.e9.5. Bracci, N.et al.(2022) Rift Valley fever virus Gn V5-epitope tagged virus enables identification of UBR4 as a Gn interacting protein that facilitates Rift Valley fever virus production.Virology. 567: 65-76.6. Wang, Y.T.et al.(2022) K48/K63-linked polyubiquitination of ATG9A by TRAF6 E3 ligase regulates oxidative stress-induced autophagy.Cell Rep. 38 (8): 110354.7. Zhu, H.et al.(2022) A comprehensive temporal patterning gene network inDrosophila.medulla neuroblasts revealed by single-cell RNA sequencing.Nat Commun. 13 (1): 1247.

技术参数

产品应用 Flow;IF

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Bio-Rad MCA1360D405GA V5-Tag抗体,mouse anti V5-TAG:Dylight®405

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