Bio-Rad MCA1283 CD88抗体,Mouse anti Human CD88-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1283 CD88抗体,Mouse anti Human CD88

2024-10-22

Mouse anti Human CD88

货号：MCA1283,MCA1283T

规格：0.2 mg,25 µg

价格：3783,893

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD88

物种：人

宿主：小鼠

抗体亚型：IgG2a

克隆号：S5/1

荧光染料：Purified

产品介绍
Mouse anti Human CD88 antibody, clone S5/1recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21).Clone S5/1 has been shown to inhibit the binding of C5a to its receptor.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Rabbit	√
Mustelid	Expected from Sequence
Bovine	√
Ferret	√
Mink	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	Pack Size: 0.2 mg 0.09% Sodium Azide (NaN3) Pack Size: 25 µg 0.09% Sodium Azide (NaN3)
Immunogen	C5aR - peptide: Met1 - Asn31.
Carrier Free	Yes
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/50	1/100
Functional Assays¹	√
Immunohistology - Frozen	X
Immunohistology - Paraffin	√
Immunoprecipitation	X
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Bio-Rad recommend the use ofMCA1283ELfor functional studies.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 5 x 105cells in 100ul.
数据
Mouse anti Human CD88 antibody, clone S5/1(MCA1283) used to stain formalin fixed, paraffin embedded human spleen sections followed by biotinylated anti Mouse IgG and streptavidin alkaline phosphatase (red). Nuclei were counterstained with hematoxylin (blue).
同型
Description	Product Code	Applications	Pack Size
Mouse IgG2a Negative Control	MCA929	F	100 Tests
文献
1. Schreiber, A.et al.(2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.J Am Soc Nephrol. 20: 289-98.2. Eglite, S.et al.(2000) Requirements for C5a receptor-mediated IL-4 and IL-13 production and leukotriene C4 generation in human basophils.J Immunol. 165: 2183-9.3. Conroy, A.et al.(2009) C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.PLoS One. 4: e4953.4. Kraft, K.et al.(2001) Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization.J Biol Chem. 276: 34408-18.5. Fukuoka, Y.et al.(2008) Generation of anaphylatoxins by human beta-tryptase from C3, C4, and C5.J Immunol. 180: 6307-16.6. Bettoni, S.et al.(2017) Interaction between Multimeric von Willebrand Factor and Complement: A Fresh Look to the Pathophysiology of Microvascular Thrombosis.J Immunol. 199 (3): 1021-40.7. Huang, L.et al.(2005) Discovery of human antibodies against the C5aR target using phage display technology.J Mol Recognit.18: 327-33.8. Tseng CWet al.(2015) Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin andStaphylococcus aureusSkin Infection.PLoS Pathog. 11 (11): e1005292.

技术参数

产品应用 Flow;IHC;FN;WB

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Bio-Rad MCA1283 CD88抗体,Mouse anti Human CD88

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