| 产品介绍 | | |
| Mouse anti Human CD42a antibody, clone GRP-Precognizes the platelet GPIX glycoprotein, a 23kDa surface marker expressed by platelets and megakaryocytes. Platelet GPIX is also known as CD42a.The CD42 complex is the major platelet receptor for von Willebrand factor. |
| 产品详情 | | |
Target Species | Human |
Species Cross-Reactivity | Target Species | Cross Reactivity |
| Mink | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Alexa Fluor® 647 - liquid |
Preparation | MCA1227A647T: Purified IgG prepared by affinity chromatography on Protein A MCA1227A647: Purified IgG prepared by ion exchange chromatography |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
Alexa Fluor®647 | 650 | 665 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| Acknowledgements | The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | 1/5 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells or 100ul whole blood |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Test |
| Human Seroblock | BUF070B | F | 200 Test |
| 数据 |
| Staining of human peripheral blood platelets with Mouse anti Human CD42a:Alexa Fluor®647 (MCA1227A647) |
| 文献 |
| 1. Harding, S.A.et al.(2007) Clopidogrel reduces platelet-leucocyte aggregation, monocyte activation and RANTES secretion in type 2 diabetes mellitus.Heart. 92: 1335-7.2. Eisbacher, M.et al.(2001) Inducible expression of the megakaryocyte-specific gene glycoprotein IX is mediated through an Ets binding site and involves upstream activation of extracellular signal-regulated kinase.Cell Growth Differ. 12: 435-45.3. Vettore, S.et al.(2008) Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant.Haematologica. 93: 1743-7.4. Fox, S.C.et al.(2004) Quantitation of platelet aggregation and microaggregate formation in whole blood by flow cytometry.Platelets. 15: 85-93.5. Din, J.N.et al.(2010) Effect of moderate walnut consumption on lipid profile, arterial stiffness and platelet activation in humans.Eur J Clin Nutr. 2011 Feb;65(2):234-9.6. Din, J.N.et al.(2008) Dietary intervention with oil rich fish reduces platelet-monocyte aggregation in man.Atherosclerosis. 197: 290-6.7. Schuberth, H.J.et al.(2007) Reactivity of cross-reacting monoclonal antibodies with canine leukocytes, platelets and erythrocytes.Vet Immunol Immunopathol. 119: 47-55.8. Tunströmer, K.et al.(2018) Quantification of Platelet Contractile Movements during Thrombus Formation.Thromb Haemost. 118 (9): 1600-11.9. Jamaly, S.et al.(2018) Elevated plasma levels of P-selectin glycoprotein ligand-1-positive microvesicles in patients with unprovoked venous thromboembolism.J Thromb Haemost. May 31 [Epub ahead of print]. |
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