Bio-Rad MCA1227F CD42a抗体,Mouse anti Human CD42a:FITC

2024-10-22

Mouse anti Human CD42a:FITC

货号:MCA1227F,MCA1227FT

规格:0.1 mg,25 µg

价格:2865,893

产品类型:流式抗体

品牌:Bio-Rad

抗原: CD42a

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:GRP-P

荧光染料:FITC

产品介绍
Mouse anti Human CD42a antibody, clone GRP-Precognizes the platelet GPIX glycoprotein, a 23kDa surface marker expressed by platelets and megakaryocytes. Platelet GPIX is also known as CD42a.The CD42 complex is the major platelet receptor for von Willebrand factor.
产品详情

Target Species

Human

Species Cross-Reactivity

Target SpeciesCross Reactivity
Mink
N.B. Antibody reactivity and working conditions may vary between species.
Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.

Preparation

MCA1227FT: Purified IgG prepared by affinity chromatography on Protein G

from tissue culture supernatant

MCA1227F: Purified IgG prepared by affinity chromatography on Protein A

from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

1% Bovine Serum Albumin

ImmunogenHuman red blood cells and platelets.
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

FITC

490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat1/5
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells or 100ul whole blood
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:FITCMCA928FF100 Tests
相关产品
DescriptionProduct CodeApplicationsPack Size
Human SeroblockBUF070AF50 Test
Human SeroblockBUF070BF200 Test
数据

Staining of human peripheral blood platelets with Mouse anti Human CD42a:FITC (MCA1227F)
文献
1. Harding, S.A.et al.(2007) Clopidogrel reduces platelet-leucocyte aggregation, monocyte activation and RANTES secretion in type 2 diabetes mellitus.Heart. 92: 1335-7.2. Eisbacher, M.et al.(2001) Inducible expression of the megakaryocyte-specific gene glycoprotein IX is mediated through an Ets binding site and involves upstream activation of extracellular signal-regulated kinase.Cell Growth Differ. 12: 435-45.3. Vettore, S.et al.(2008) Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant.Haematologica. 93: 1743-7.4. Fox, S.C.et al.(2004) Quantitation of platelet aggregation and microaggregate formation in whole blood by flow cytometry.Platelets. 15: 85-93.5. Din, J.N.et al.(2010) Effect of moderate walnut consumption on lipid profile, arterial stiffness and platelet activation in humans.Eur J Clin Nutr. 2011 Feb;65(2):234-9.6. Din, J.N.et al.(2008) Dietary intervention with oil rich fish reduces platelet-monocyte aggregation in man.Atherosclerosis. 197: 290-6.7. Schuberth, H.J.et al.(2007) Reactivity of cross-reacting monoclonal antibodies with canine leukocytes, platelets and erythrocytes.Vet Immunol Immunopathol. 119: 47-55.8. Tunströmer, K.et al.(2018) Quantification of Platelet Contractile Movements during Thrombus Formation.Thromb Haemost. 118 (9): 1600-11.9. Jamaly, S.et al.(2018) Elevated plasma levels of P-selectin glycoprotein ligand-1-positive microvesicles in patients with unprovoked venous thromboembolism.J Thromb Haemost. May 31 [Epub ahead of print].

技术参数
© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://gibcohyclone.cn/thread-41036.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价