Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX33,Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX33,Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2

2024-10-22

Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2

货号：MCA1044F

规格：100 Tests

价格：3783

产品类型：流式抗体

品牌：Bio-Rad

抗原：MHC Class II Monomorphic

物种：人

宿主：大鼠

抗体亚型：IgG2a

克隆号：YKIX33

荧光染料：FITC

产品介绍
Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobboldet al.1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti Dog MHC Class II immunoprecipitates an antigen of ~32/34 kDa and blocks the proliferation of MHC Class II dependent responsesin vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
产品详情
Target Species	Dog
Species Cross-Reactivity	Target Species	Cross Reactivity
Hooded Seal	×
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Immunogen	Con A activated canine peripheral blood cells.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
产品推荐
Description	Product Code	Applications	Pack Size
Rat IgG2a Negative Control:FITC	MCA1212F	F	100Tests
文献
1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX33,Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2

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