Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX33,Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2

2024-10-22

Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2

货号:MCA1044GA

规格:0.1 mg

价格:2185

产品类型:流式抗体

品牌:Bio-Rad

抗原:MHC Class II Monomorphic

物种:dog

宿主:大鼠

抗体亚型:IgG2a

克隆号:YKIX334.2

荧光染料:Purified

产品介绍
Rat anti Dog MHC Class II Monomorphic antibody, clone YKIX334.2recognizes a monomorphic epitope on canine MHC Class II and was classified at the First Canine Leucocyte Antigen Workshop (Cobboldet al.1994).The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In dogs, this is referred to as the dog leukocyte antigen (DLA) region. Rat anti Dog MHC Class II immunoprecipitates an antigen of ~32/34 kDa and blocks the proliferation of MHC Class II dependent responsesin vitro. In dogs, MHC Class II is expressed by all peripheral blood mononuclear cells.
产品详情
Target SpeciesDog
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
Carrier FreeYes
ImmunogenCon A activated canine peripheral blood cells.
Approx. Protein ConcentrationsIgG concentration 1 mg/ml
Fusion PartnersSpleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/501/100
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
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文献
1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.

技术参数

产品应用 Flow;IHC;IP

产品类型 单克隆抗体

推荐用于 Flow;IHC;IP

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