Rat anti Dog CD45R antibody, clone YKIX753.22.2,Rat anti Dog CD45R antibody, clone YKIX753.22.2

2024-10-22

Rat anti Dog CD45R antibody, clone YKIX753.22.2

货号:MCA1043PE

规格:100 Tests

价格:4250

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD45R

物种:dog

宿主:大鼠

抗体亚型:IgG2b

克隆号:YKIX753.22.2

荧光染料:PE

产品介绍
Rat anti Dog CD45R antibody, clone YKIX753.22.2recognizes canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220 kDa from Con A Blasts, (Cobboldet al.1994). It is expressed on subsets of leucocytes in canine blood of all outbred mongrels and beagles tested. Rat anti dog CD45R, clone YKIX753.22.2 binds to antigen immunoprecipitated by the Rat anti Dog CD45 antibodyclone YKIX716.13(Cobboldet al.1994).
产品详情
Target SpeciesDog
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
ReconstitutionReconstitute with 1ml distilled water
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
ImmunogenCanine thymocytes
Approx. Protein ConcentrationsIgG concentration 0.05mg/ml
Fusion PartnersSpleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
产品推荐
DescriptionProduct CodeApplicationsPack Size
Rat IgG2b Negative Control:RPEMCA6006PEF100 Tests
文献
1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.

技术参数

产品应用 Flow

产品类型 单克隆抗体

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