c-Myc (Total) Human ELISA Kit/人c-Myc(总)ELISA试剂盒
货号:KHO2041
规格:96 tests
价格:8160
产品类型:Platinum ELISA
品牌:Thermo Fisher
物种:人
宿主:其它
抗体亚型:其它
荧光染料:其它
灵敏度 | <5 pg/mL |
检测范围 | 15.6-1000 pg/mL |
样本类型/体积 | 细胞裂解液 5μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr |
Homogenous (no wash) | No |
批间变异系数(CV) | 3.7% |
批内变异系数(CV) | 4.9% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 标准稀释液 - 检测抗体 - 抗兔HRP - HRP稀释剂 - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
Protein name | c-Myc |
Protein aliases | avian myelocytomatosis viral oncogene homolog, bHLHe39, Class E basic helix-loop-helix protein 39, Myc proto-oncogene protein, myc-related translation/localization regulatory factor, Proto-oncogene c-Myc, Transcription factor p64, v-myc myelocytomatosis viral oncogene homolog |
物种(Tested) | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记(染料) | HRP |
检测抗体偶联物 | HRP |
Gene aliases | BHLHE39, c-Myc, MRTL, MYC, MYCC |
Gene ID | (Human) 4609 |
Gene symbol | MYC |
UniProt ID | (Human) P01106 |
The Human c-Myc Total (Hu c-Myc) ELISA quantitates Hu c-Myc in human cell lysates. The assay will exclusively recognize both natural and recombinant Hu c-Myc, independed of phosphorylation state.人c-Myc总量(人c-Myc)ELISA定量了人细胞裂解物中的人c-Myc。 该检测试剂盒将专门识别天然和重组人c-Myc,而与磷酸化状态无关。Principle of the method 方法原理The Human c-Myc solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人c-Myc固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.c-Myc蛋白是一个62 kDa的转录因子,由人类染色体8q24上的c-Myc基因编码。 c-Myc通常在多种肿瘤细胞中被激活,并在细胞增殖,分化,凋亡和细胞周期进程中发挥重要作用。已经研究了c-Myc的磷酸化,先前的研究表明糖原合酶激酶3,细胞周期蛋白依赖性激酶,ERK2和C-Jun N末端激酶(JNK)在Thr58 / Ser62的磷酸化与细胞增殖和细胞周期之间存在功能关联。研究表明,c-Myc对于肿瘤发展中的血管生成和血管生成必不可少,该过程将血液分配到整个细胞中。 c-myc癌基因(p62 c-myc)参与正常细胞增殖和分化的控制,而c-Myc表达失调会诱导不同细胞类型的凋亡。针对c-myc表位的抗体识别含有与目标蛋白的氨基或羧基末端融合的Myc表位标签的过表达蛋白。 c-Myc,N-Myc和L-Myc编码的蛋白质在细胞增殖,分化和肿瘤疾病中起作用。在几种类型的人类肿瘤中发现了c-Myc基因的扩增,包括肺癌,乳腺癌和结肠癌,而在神经母细胞瘤中发现了N-Myc基因的扩增。 8号染色体上的c-myc基因座易位至14号染色体上的免疫球蛋白基因座(重链); 2(三角轻链); Burkitts淋巴瘤和其他B细胞淋巴增生性疾病中描述了22或22(轻链)。 c-myc基因的异常表达发生在不同来源的肿瘤中,例如大肠癌,胃癌,胆囊癌,肝癌,乳腺癌,卵巢癌,子宫内膜癌,头颈癌,肺癌,前列腺癌,甲状腺癌,口腔癌,眼癌,鼻咽癌,内分泌癌。以及造血肿瘤。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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