FAM FLICA™ Caspase-10 Kit ICT923 BIO-RAD,FAM FLICA™ Caspase-10 Kit

2024-10-22

FAM FLICA™ Caspase-10 Kit

货号:ICT923

规格:100 Tests

价格:7591

产品类型:细胞凋亡检测

品牌:Bio-Rad

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

产品介绍
The FAM FLICA caspase-10 kituses a quick and easy method to analyze active caspases in apoptotic cells.
产品详情
Reagents in the Kit4 vials of FAM-AEVD-FMK FLICA Reagent - lyophilized10x Apoptosis Wash Buffer, 60 mLFixative, 6 mLPropidium Iodide, 1 mLHoechst 33342, 1 mL
Test PrincipleCaspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.The Caspase FLICA kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FAM494520
AcknowledgementsFLICA™ is a trademark of Immunochemistry Technologies, LLC.
RegulatoryFor research purposes only
GuaranteeGuaranteed until date of expiry. Please see product label.
存储条件
Store the unopened kit and each unopened component at +4℃until the expiration date.Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20℃for 6 months protected from light and thawed no more than twice during that time.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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产品类型 细胞凋亡检测

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