Human Anti-Pembrolizumab Antibody, clone AbD30689 HCA296 ,Human Anti-Pembrolizumab Antibody, clone AbD30689

2024-10-22

Human Anti-Pembrolizumab Antibody, clone AbD30689

货号:HCA296

规格:0.1 mg

价格:3851

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Pembrolizumab

物种:其它

宿主:人

抗体亚型:IgG1

克隆号:AbD30689

荧光染料:其它

产品介绍
Human Anti-Pembrolizumab Antibody, clone AbD30689is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the free humanized monoclonal antibody pembrolizumab. The antibody does not recognize recombinant human programmed cell death 1 (PD-1) or pembrolizumab in complex with recombinant human PD-1 and can be used to measure free pembrolizumab levels in serum from patients.A pair of anti-pembrolizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with an HRP conjugated Anti-Pembrolizumab Antibody in full immunoglobulin format, clone AbD30685_hIgG1 (HCA297) as the detection antibody.Pembrolizumab (Keytruda) is a humanized (IgG4/kappa) antibody with the heavy chain mutation S228P (IgG4-Pro). Pembrolizumab is used to treat inoperable or metastatic melanoma, metastatic non-small cell lung cancer (NSCLC), and as a second-line treatment for head and neck squamous cell carcinoma (HNSCC). Pembrolizumab is a first line treatment for NSCLC if the cancer overexpresses PDL1 and has no mutations in EGFR or ALK. Pembrolizumab is a checkpoint inhibitor and acts as an immunomodulator by blocking ligand activation of PD-1 receptor on T cells, thereby activating T cells to attack the cancer. It blocks PD-1 interaction with PD-1 ligand (PD-L1) and PD-1 ligand 2 (PD-L2).
产品详情
Product FormA monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH). This antibody is supplied as a liquid.
PreparationMetal chelate affinity chromatography
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.01% Thiomersal
AffinityThe monovalent intrinsic affinity of AbD30689 was measured as KD= 0.6 nM by real time, label free molecular interaction analysis on immobilized pembrolizumab
ImmunogenPembrolizumab
Approx. Protein ConcePurityntrationsAntibody concentration 0.5 mg/ml
AffinityThe monovalent intrinsic affinity of AbD30689 was measured as KD= 0.6 nM by real time, label free molecular interaction analysis on immobilized pembrolizumab
AcknowledgementsThis product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.Keytruda is a trademark of Merck & Co.His-tag is a registered trademark of EMD Biosciences.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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技术参数

产品应用 ELISA

产品类型 单克隆抗体

推荐用于 ELISA

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