H3K18ac Polyclonal Antibody/H3K18ac多克隆抗体

货号：720095

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Acetylated peptide (Lys18) corresponding to Human

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 µg/mL(ICC)；0.5 µg/mL(IF)；1-2 µg/mL(WB)；0.25 µg/mL(Array)

产品详细信息These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.Since it is conserved across species, the antibody may react with many other species.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K18ac Antibody (720095) in IFImmunofluorescence was performed on fixed and permeabilized HeLa cells for detection of Histone H3K18ac using Anti-Histone H3K18ac Rabbit Polyclonal Antibody (Product # 720095, 0.5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:400). Panel a) shows representative cells that were stained for detection and localization of Histone H3K18ac protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938,). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Histone H3K18ac. Panel e) represents control cells with no primary Antibody to assess background. H3K18ac Antibody (720095) in WBWestern blot analysis was performed on nuclear enriched cell extracts (30 µg lysate) of HeLa (Lane1), HeLa treated with SAHA (0.5 uM/24 hrs) (Lane 2), HeLa treated with Sodium butyrate (5mM/24 hours) (Lane 3), HCT 116 (Lane 4), HCT 116 treated with SAHA (0.5 uM/24 hrs) (Lane 5), HCT 116 treated with Sodium butyrate (5mM/24 hrs) (Lane 6), Jurkat (Lane 7), Jurkat treated with SAHA (0.5 uM/24 hours) (Lane 8), Jurkat treated with Sodium butyrate (5mM/24 hours) (Lane 9) and K562 (Lane 10) and K562 treated with SAHA (0.5 uM/24 hrs) (Lane 11). The blot was probed with Anti-Histone H3K18ac Rabbit Polyclonal Antibody (Product # 720095, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 17kDa band corresponding to Histone H3K18ac was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemilumines

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-46413.htm