Human Anti-Golimumab Antibody, clone AbD25455 HCA289 产品推荐,Human Anti-Golimumab Antibody, clone AbD25455-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Human Anti-Golimumab Antibody, clone AbD25455 HCA289 产品推荐,Human Anti-Golimumab Antibody, clone AbD25455

2024-10-22

Human Anti-Golimumab Antibody, clone AbD25455

货号：HCA289

规格：0.1 mg

价格：4446

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Golimumab

物种：其它

宿主：人

抗体亚型：其它

克隆号：AbD25455

荧光染料：其它

产品介绍
Human Anti-Golimumab Antibody, clone AbD25455is an anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug golimumab. This antibody binds to both free golimumab and to golimumab bound to its target tumor necrosis factor alpha (TNFα). Clone AbD25455 does not inhibit the binding of golimumab to its target TNFα and can be used to measure total golimumab and biosimilar products in bioanalytical assays.Clone AbD25455 is an affinity-matured variant of clone AbD20897 (HCA242), with about 7-fold affinity improvement.A pair of anti golimumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with an antibody in full immunoglobulin format, clone AbD25451_hIgG1 (HCA290), as the detection antibody.Golimumab, marketed under the brand name Simponi is a recombinant human IgG1/kappa monoclonal antibody approved for the treatment of rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. This therapeutic antibody, directed against TNFα, acts by blocking the binding of TNFα to its receptors, resulting in a down-regulation of the inflammatory response associated with autoimmune diseases.
产品详情
Product Form	A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed inE. coli.The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation	Metal chelate affinity chromatography
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.01% Thiomersal
Immunogen	Recombinant human IgG1 kappa
Approx. Protein ConcePurityntrations	IgG concentration 0.5 mg/ml
Acknowledgements	This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.Simponi is a registered trademark of Janssen Biotech, Inc. in the USA and Merck & Co, Inc. in Europe.His-tag is a registered trademark of EMD Biosciences.
Affinity	The monovalent intrinsic affinity of AbD25455 was measured as KD= 0.9 nM by real time, label free molecular interaction analysis on immobilized golimumab
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	1/100	1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
产品推荐
Description	Product Code	Applications	Pack Size
Human anti Golimumab	HCA290	E	0.1mg
Human anti Golimumab:HRP	HCA290P	E	0.1mg

技术参数

产品应用 elisa

产品类型单克隆抗体

推荐用于 elisa

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Human Anti-Golimumab Antibody, clone AbD25455 HCA289 产品推荐,Human Anti-Golimumab Antibody, clone AbD25455

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