HuCAL Fab-Max-FH negative control, clone AbD24004 HCA269 bio-rad,HuCAL Fab-Max-FH negative control, clone AbD24004

2024-10-22

HuCAL Fab-Max-FH negative control, clone AbD24004

货号:HCA269

规格:0.1 mg

价格:1896

产品类型:其它同型对照

品牌:Bio-Rad

物种:人

宿主:其它

抗体亚型:其它

克隆号: AbD24004

荧光染料:其它

产品介绍
HuCAL Fab-Max-FH negative control, clone AbD24004is a recombinant Fab antibody fragment in the format Fab-Max-FH with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It has been tested by ELISA for specificity to GFP, but has not been tested in other applications. It is suggested as a negative control reagent when using other HuCAL antibodies of the same Fab format.It is recommended that this reagent is used at the same concentration as the test reagent.
产品详情
Target SpeciesNegative Control
Product FormA bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed inE. coli.This Fab fragment is bivalent by dimerization of an inactive variant of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
PreparationMetal chelate affinity chromatography
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.01% Thiomersal
ImmunogenGreen Fluorescent Protein (GFP)
Approx. Protein ConcePurityntrationsAntibody concentration 0.5 mg/ml
AcknowledgementsThis product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.His-tag is a registered trademark of EMD Biosciences.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Rhodes, S.G.et al.(2014) Use of antigen-specific interleukin-2 to differentiate between cattle vaccinated with Mycobacterium bovis BCG and cattle infected with M. bovis.Clin Vaccine Immunol. 21:39-45.

技术参数

产品应用 elisa

产品类型 同型对照

推荐用于 elisa

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