Human anti Bovine Interleukin-2, clone AbD22297 HCA268 bio-rad ,Human anti Bovine Interleukin-2, clone AbD22297-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Human anti Bovine Interleukin-2, clone AbD22297 HCA268 bio-rad ,Human anti Bovine Interleukin-2, clone AbD22297

2024-10-22

Human anti Bovine Interleukin-2, clone AbD22297

货号：HCA268

规格：0.1 mg

价格：2151

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Bovine Interleukin-2

物种：人

宿主：人

抗体亚型：其它

克隆号：AbD22297

荧光染料：Purified

产品介绍
Human anti Bovine Interleukin-2, clone AbD22297recognizes bovine interleukin-2 (IL-2), also known as T-cell growth factor (TCGF). Bovine IL-2 is a 155 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation.Human anti bovine interleukin-2, clone AbD22297, has been successfully used for the detection and measurement of native and recombinant bovine IL-2 in ELISA where it acts as the detection antibody in a sensitive assay for bovine IL-2 determination. Bio-Rad recommend clone AbD21766 (HCA267) for intracellular flow cytometry.
产品详情
Target Species	Bovine
Product Form	A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed inE. coli.This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a V5 tag and a double extended Strep-tag (SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK).
Preparation	Metal chelate affinity chromatography
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.01% Thiomersal
Immunogen	Fc-fusion protein containing the amino acid sequence 21-155 from bovine interleukin-2
Affinity	The monovalent intrinsic affinity of this antibody was measured as KD=0.3 nM by real time, label-free molecular interaction analysis on recombinant bovine IL-2.
Purity	Purity was tested by SDS-PAGE and Coomassie-staining of a 2 μg sample.
Approx. Protein ConcePurityntrations	Antibody concentration 0.5 mg/ml
Acknowledgements	This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
ELISpot	√
Flow Cytometry	×
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Rhodes, S.G.et al.(2014) Use of antigen-specific interleukin-2 to differentiate between cattle vaccinated with Mycobacterium bovis BCG and cattle infected with M. bovis.Clin Vaccine Immunol. 21:39-45.

技术参数

产品应用 elisa

产品类型单克隆抗体

推荐用于 elisa

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Human anti Bovine Interleukin-2, clone AbD22297 HCA268 bio-rad ,Human anti Bovine Interleukin-2, clone AbD22297

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