Human anti Human HbA1c antibody, clone AbD15783_IgG1 BIO-RAD ,Human anti Human HbA1c antibody, clone AbD15783_IgG1

2024-10-22

Human anti Human HbA1c antibody, clone AbD15783_IgG1

货号:HCA197

规格:0.1 mg

价格:2576

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:HbA1c

物种:其它

宿主:人

抗体亚型:IgG1

克隆号: AbD15783_IgG1

荧光染料:Purified

产品介绍
Human anti Human HbA1c antibody, clone AbD15783_IgG1recognizes HbA1c, hemoglobin glycated at the N-terminal valine of the hemoglobin β chain. It does not bind to non-glycated hemoglobin. Besides binding to the normal glycated hemoglobin, Human anti Human HbA1c antibody, clone AbD15783_IgG1 also recognizes the glycated hemoglobin variants HbC, HbE, HbD and to a lesser degree HbS. Elevated HbF is not detected due to the absence of the hemoglobin β chain.The measurement of HbA1c levels is used to identify the average plasma glucose concentration. In diabetes mellitus, higher levels of HbA1c have been associated with cardiovascular disease and other diseases. Levels of more than 6.5% HbA1c (48 mmol/mol) are used as a criterion to diagnose diabetes. This antibody is also available in a Fab format (HCA179).
产品详情
Target SpeciesHuman
Product FormHuman IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line.This antibody is supplied as a liquid.
PreparationPurified IgG prepared by affinity chromatography on Protein A
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.01% Thiomersal
ImmunogenThe immunogens used in the generation of this antibody were:A glycated peptide, corresponding to N-terminal 9 amino acids of haemoglobin beta chain coupled to the carrier proteins BSA and TRFPurified HbA1c
AffinityThe affinity of this antibody, as a monovalent fab measured against the immunogen, was measured as KD=19 nM by real time, label-free molecular interaction analysis using HbA1c-BSA and HbA1c-TRF conjugates as immobilized antigen.The affinity of this antibody, as a monovalent fab measured against purified HbA1c, was measured as KD=58 nM by real time, label-free molecular interaction analysis using full length HbA1c as immobilized antigen
Approx. Protein ConcePurityntrationsTotal protein concentration 0.5 mg/ml
AcknowledgementsThis product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
产品推荐
DescriptionProduct CodeApplicationsPack Size
Human anti Human HbA1cHCA179E0.1 mg

技术参数

产品应用 ELISA

产品类型 单克隆抗体

推荐用于 ELISA

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