| 产品介绍 | | |
| Human anti cyclophilin A antibody, clone AbD00794recognizes cyclophilin A, also known as peptidyl-prolyl cis/trans isomerase A, PPIA or rotamase A. Cyclophilin A is a ~18 kDa, ubiquitously distributed intracellular protein that can be secreted by cells in response to inflammatory stimuli (Jinet al.2000).Cyclophilin A is a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and, therefore, are believed to be involved in protein folding and/or intracellular protein transport (Fischeret al.1989). This protein can interact with several HIV proteins including p55 gag, Vpr, and capsid protein. It has been shown to be necessary for the formation of infectious HIV virions (Frankeet al.1994). Cyclophilin is a specific high-affinity binding protein for the immunosuppressant agent cyclosporin A (Handschumacheret al.1984). Because of its dramatic effects on decreasing morbidity and increasing survival rates in human transplants, the molecular mechanism of immunosuppression by cyclosporin A has been a matter of much interest (Duretteet al.1988). Multiple PPIA pseudogenes that map to different chromosomes have been reported. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed (Chatellard-Gruaz Det al.1994).Human anti cyclophilin antibody, clone AbD00794 has been used successfully for the detection of cyclophilin A in HeLa cell lysates by Western blotting. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL®GOLD phage display library. Expressed inE. coliand purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. |
| Preparation | Metal-chelate affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Full length human recombinant cyclophilin A, amino acid residues 1-164, molecular weight 17.9 kDa |
| Activity | Activity was tested by indirect ELISA: recombinant purified Cyclophilin A (5 μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA005 (2 μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate. 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above background. |
| Purity | Purity was tested by SDS-PAGE and Coomassie-staining of a 2 μg sample. |
| Approx. Protein ConcePurityntrations | Antibody concentration 0.5 mg/ml |
| Acknowledgements | This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.His-tag is a registered trademark of EMD Biosciences. |
| Regulatory | For research purposes only |
| Guarantee | 6 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | 2ug/ml |
| Western Blotting | √ | | 0.5ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E,WB | 0.5 ml |
| Goat anti Human IgG F(ab')2:Alk. Phos. | 0500-0100 | E,WB | 0.5 ml |
QQ:1749072012