| 产品介绍 | | |
| Human anti Human CD292 antibody, clone AbD01564recognizes human CD292, also known as bone morphogenetic protein receptor type 1A (BMPR-1A) and does not cross-react with human BMPR-1B.The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR-1A and BMPR-1B and the type II receptor BMPR2.These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. BMPR-1A is the receptor for BMP-2 and BMP-4. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kDa and type II receptors of about 70-80 kDa. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signalling, whereas type I receptors require their respective type II receptors for ligand binding.In humans, mutations in the BMPR-1A gene are responsible for juvenile polyposis syndrome, juvenile intestinal polyposis, and Cowden disease. Human anti Human CD292 antibody inhibits BMP-2 mediated stimulation of C2C12 cells. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Monovalent human recombinant Fab (lambda light chain) selected from the HuCAL®GOLD phage display library. Expressed inE. coliand purified using Streptactin affinity chromatography. The antibody is tagged with a strepII-tag (NWSHPQFEK) at the C-terminus of the antibody heavy chain-liquid. |
| Preparation | Streptactin affinity chromatography. |
| Buffer Solution | Borate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Immunogen | Human BMPR-1A extracellular domain, recombinant expressed inE. coli(amino acid residues 24-152, molecular weight 14.2 kDa) |
| Activity | Activity was tested by indirect ELISA: recombinant purified BMPR-1A (5 μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA004 (2 μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate, 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above back-ground. |
| Purity | Purity was tested by SDS-PAGE and Coomassie-staining of a 2 μg sample. |
| Affinity | kD = 2.2 nMThe monovalent intrinsic affinity of HCA004 was measured by BIAcore on the immobilized BMPR-1A extracellular domain. |
| Approx. Protein ConcePurityntrations | Antibody concentration 0.5 mg/ml. |
| Acknowledgements | This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.Blot data kindly provided by Professor Sebald, University of Wuerzburg, Germany. |
| Regulatory | For research purposes only |
| Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | | 2ug/ml |
| Functional Assays | √ | | |
| Western Blotting | √ | | 2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E,WB | 0.5 ml |
| Goat anti Human IgG F(ab')2:Alk. Phos. | 0500-0100 | E,WB | 0.5 ml |
| 文献 |
| 1.Harth, S.et al.(2010) A selection fit mechanism in BMP receptor IA as a possible source for BMP ligand-receptor promiscuity.PLoS One. 5(9). pii: e13049.2.Harth, S.et al.(2010) Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.Acta Crystallogr Sect F Struct Biol Cryst Commun. 66: 964-8. |
QQ:1749072012