Goat anti green fluorescent protein antibody AHP975 bio-rad,Goat anti green fluorescent protein antibody

2024-10-22

Goat anti green fluorescent protein antibody

货号:AHP975

规格:0.1 mg

价格:1598

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:green fluorescent protein

物种:其它

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍
Goat anti green fluorescent protein antibodyrecognizes green fluorescent protein (GFP), a 27kD protein which is derived from the jellyfishAequorea victoria. GFP fluoresces green (509nm) when excited by blue light (395nm) and is commonly used as a marker of gene expression. The antibody recognises wild type GFP, but will also detect recombinant and enhanced forms of GFP.
产品详情
Product FormPurified IgG - liquid
Antiserum PreparationAntisera to green fluorescent protein (GFP) were raised by repeated immunisations of goat with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers<0.1% Sodium Azide (NaN3)
ImmunogenFusion protein corresponding to full length GFP derived fromAequorea victoria
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA1/10,0001/30,000
Immunofluorescence
Western Blotting1/4001/10,000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Rabbit anti Goat IgG (Fc):FITCSTAR122FF100 Tests
Rabbit anti Goat IgG (Fc):HRPSTAR122PC,E,WB100 Tests
文献
1.Wen, J, & Brogna, S. (2010) Splicing-dependent NMD does not require the EJC inSchizosaccharomyces pombe.EMBO J. 29: 1537-51.2.Wuest, T.et al.(2011) The Herpes Simplex Virus-1 Transactivator Infected Cell Protein-4 Drives VEGF-A Dependent Neovascularization.PLoS Pathog. 7: e1002278.3.Al-Jubran, K.et al.(2013) Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus.RNA. 19: 1669-83.4.Lampron, A.et al.(2013) Migration of bone marrow-derived cells into the central nervous system in models of neurodegeneration.J Comp Neurol. 521 (17): 3863-76.5.Nicol, M.Q.et al.(2019) Lack of IFNγ signaling attenuates spread of influenza A virus in vivo and leads to reduced pathogenesis.Virology. 526: 155-164.

技术参数

产品应用 wb,elisa

产品类型 多克隆抗体

推荐用于 wb,elisa

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