JMJD1A Polyclonal Antibody

货号：PA5-88279

规格：100 µL

价格：4732

产品类型：标签抗体

品牌：Thermo Fisher

抗原：A synthetic peptide corresponding to a sequence wi

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1.13 mg/mL	用法：	1:50-1:200（ICC）；1:50-1:200（IF）；1:50-1:200（IHC）；1:500-1:2000(WB)

产品详细信息Immunogen sequence: LNTKSEALRT KPDVCKAGLL SKSSQIGTGD LKILTEPKGS CTQPKTNTDQ ENRLESVPQA LTGLPKECLP TKASSKAELE IANPPELQKH LEHAPSPSDV S; Positive Samples: 293T; Cellular Location: Cytoplasm, Nucleus靶标信息JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

数据：

JMJD1A Antibody (PA5-88279) in ICCImmunocytochemistry-Immunofluorescence analysis of JMJD1A was performed in NIH-3T3 cells using JMJD1A Polyclonal Antibody (Product # PA5-88279) at a dilution of 1:100. Blue: DAPI for nuclear staining. JMJD1A Antibody (PA5-88279) in IHCImmunohistochemistry analysis of JMJD1A in paraffin-embedded mouse testis using JMJD1A Polyclonal Antibody (Product # PA5-88279) at a dilution of 1:100. JMJD1A Antibody (PA5-88279) in WBWestern Blot analysis of JMJD1A in extracts of 293T cells using JMJD1A Polyclonal Antibody (Product # PA5-88279) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

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