Rabbit anti Human caspase-7 antibody AHP966 bio-rad,Rabbit anti Human caspase-7 antibody

2024-10-22

Rabbit anti Human caspase-7 antibody

货号:AHP966

规格:0.1 mg

价格:3672

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:caspase-7

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human caspase-7 antibodyrecognizes an epitope within the C-terminal region (CT) of Caspase-7, a ~35 kDa cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3).Caspase-7, a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumour necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADP-ribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and granzyme-B mediated apoptosis pathways.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationAntisera to human Caspase-7 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.02%Sodium Azide
ImmunogenA 16 amino acid peptide from human Caspase-7 carboxy-terminus
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Western Blotting1.0ug/ml2.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Duan, H.et al.(1996) ICE-LAP3, a novel mammalian homologue of theCaenorhabditis eleganscell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis.J Biol Chem. 271 (3): 1621-5.2.Juan, T.S.et al.(1997) Identification and mapping of Casp7, a cysteine protease resembling CPP32 beta, interleukin-1 beta converting enzyme, and CED-3.Genomics. 40 (1): 86-93.3.Cohen, G.M. (1997) Caspases: the executioners of apoptosis.Biochem J. 326 ( Pt 1): 1-16.

技术参数

产品应用 wb

产品类型 多克隆抗体

推荐用于 wb

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