Rabbit anti Endonuclease G antibody AHP730 bio-rad ,Rabbit anti Endonuclease G antibody

2024-10-22

Rabbit anti Endonuclease G antibody

货号:AHP730

规格:0.1 mg

价格:5287

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Endonuclease G

物种:其它

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Endonuclease G antibodyrecognizes human endonuclease G, also known as endoG. Endo G is a 297 amino acid, including a 48 amino acid transit peptide ~36 kDa mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis.EndoG is reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria (Liet al.2001).
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
Buffer SolutionPhosphate buffered saline
ImmunogenSynthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Antiserum PreparationAntisera to endonuclease G were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Preservative Stabilisers0.02%Sodium Azide
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining alquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Immunohistology - Paraffin115g/ml
Western Blotting1ug/ml2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Colado, E.et al.(2011) Zalypsis has in vitro activity in acute myeloid blasts and leukemic progenitor cells through the induction of a DNA damage response.Haematologica. 96: 687-95.2.Amoury, M.et al.(2016) A novel approach for targeted elimination of CSPG4-positive triple-negative breast cancer cells using a MAP tau-based fusion protein.Int J Cancer. 139 (4): 916-27.3.Maiso, P.et al.(2008) The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis in multiple myeloma cells.Br J Haematol. 141 (4): 470-82.

技术参数

产品应用 wb

产品类型 Polyclonal Antibody

推荐用于 wb

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