Bio-Rad IWNT-3抗体 AHP2268,Goat anti WNT-3-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad IWNT-3抗体 AHP2268,Goat anti WNT-3

2024-10-22

Goat anti WNT-3

货号：AHP2268

规格：0.1 ml

价格：5763

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：IWNT-3

物种：人

宿主：山羊

抗体亚型：IgG

荧光染料：Purified

产品介绍
Goat anti Wnt-3 antibodyrecognises both human and mouse proto-oncogene Wnt-3, a member of the Wnt family, and ligand of the frizzled family of seven transmembrane receptors.Wnt-3, otherwise known as Int-4, is involved in cell fate decisions and patterning during embryogenesis, cell signalling during neural tube development, and implicated in the tumorogenesis of several cancers including breast, lung, hepatocellular(HCC), and gastric carcinoma.Over-expression of Wnt-3 playes a pivotal role in the resistance of HER2-overexpressing breast tumors to the breast cancer treatment drug Trastuzumab, also known as Herceptin (Wuet al.2012). The effects of Wnt-3 over-expression included the transactivated expression of EGFR (epidermal growth factor receptor) an increased expression of B-catenin within the nucleus, an increase in N-cadherin and the neural crest transcription factor SLUG, as well as promoting an EMT (epithelial to mesenchymal transition) response.Goat anti Wnt-3 polyclonal antibody exhibits strong fibrous staining in myoepithelial cells of human breast tissue in paraffin sections.Goat anti Wnt-3 polyclonal antibody stains mouse hippocampal primary astrocytes in ICC/IF (Okamotoet al.2011).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species Cross Reactivity
	Mouse √
	Dog Expected from Sequence
	Rat Expected from Sequence
	N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
AntiserumPreparation	Antiserum to Wnt-3 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide
Buffer Solution	TRIS buffered saline
Preservative Stabilisers	0.02% Sodium Azide (NaN₃)0.5% Bovine Serum Albumin
Immunogen	Synthetic peptide sequence C-GRGHNTRTEKRKEK from the internal region of Wnt-3 (NP_110380.1).
Approx. Protein Concentrations	IgG concentration 0.5 mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√		1:16000
Immunofluorescence	√
Immunohistology - Paraffin¹	√	3.0	5.0ug/ml
Western Blotting	√	1.0	3.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Histology Positive Control Tissue Human breast Western Blotting AHP2268 detects a band of approximately 40kDa in HEK293 cell lysates
二抗相关产品
Description	Product Code	Applications	Pack Size
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg
相关产品
Description	Product Code	Applications	Pack Size
Antigen Retrieval Buffer, pH8.0	BUF025A	p	500 ml
文献
Further Reading1.Wu, Y.et al.(2012) Expression of Wnt3 activates Wnt/β-catenin pathway and promotes EMT-like phenotype in trastuzumab-resistant HER2-overexpressing breast cancer cells.Mol Cancer Res. 10 (12): 1597-606.

技术参数

产品应用 E ;IF ;P* ;WB

产品类型 Polyclonal Antibody

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Bio-Rad IWNT-3抗体 AHP2268,Goat anti WNT-3

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