产品介绍 |
| Goat anti Human furin antibodyrecognizes an epitope corresponding to aa553-565 of human Furin, otherwise known as PACE (Paired basic Amino acid Cleaving Enzyme). Furin is a calcium-dependent serine endoprotease and proprotein convertase, which plays a crucial role in the processing of latent precursor proteins into their biologically active forms, such as during the Notch signaling pathway.The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T cell development, and both tumor progression and suppression.Furin is a critical enzyme during the S1 cleavage of pre-Notch proteins within the golgi apparatus, to convert the nascent form into the mature heterodimeric Notch receptor and the non-covalently associated intracellular domain. These are then transported to the cell surface, in preparation for ligand-receptor binding and further S2 and S3 cleavage, resulting in the release and translocation of the intracellular domain to the nucleus. |
产品详情 |
Target Species | Human |
Species Cross-Reactivity | Target Species Cross Reactivity |
| Mouse Expected from Sequence |
| Rat Expected from Sequence |
| Dog Expected from Sequence |
| Pig Expected from Sequence |
| Bovine Expected from Sequence |
| N.B. Antibody reactivity and working conditions may vary between species. |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to human Furin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3)0.5% Bovine Serum Albumin |
Immunogen | Synthetic peptide sequence C-NTSEANNYGTLTK from the internal region of Furin (NP_002560.1). |
Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | 1/8000 |
Immunohistology - Paraffin1 | √ | 2.0 | 4.0ug/ml |
Western Blotting | × | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- Histology Positive Control Tissue
- Human liver
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Description | Product Code | Applications | Pack Size |
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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相关产品 |
Description | Product Code | Applications | Pack Size |
Antigen Retrieval Buffer, pH8.0 | BUF025A | p | 500 ml |
文献 |
| Further Reading1.Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.Nat Rev Mol Cell Biol. 7 (9): 678-89. |
QQ:1749072012