Bio-Rad MIB1抗体 AHP2264,Goat anti Human MIB1-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MIB1抗体 AHP2264,Goat anti Human MIB1

2024-10-22

Goat anti Human MIB1

货号：AHP2264

规格：0.1 mg

价格：3383

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：MIB1

物种：人

宿主：山羊

抗体亚型：IgG

荧光染料：Purified

产品介绍
Goat anti Human MIB-1 antibodyrecognizes human E3 ubiquitin-protein ligase MIB1, also known as Mind bomb homolog 1, DAPK-interacting protein 1 or Zinc finger ZZ type with ankyrin repeat domain protein 2, encoded by the MIB1 gene. E3 ubiquitin-protein ligase MIB1 is a 1006 amino acid ~130 kDa E3 ubiquitin-protein ligase which plays a role in the regulation of apoptosis through DAPK interaction, and also a key role in the positive regulation of the Notch signaling pathway.MIB1 mediates the ubiquitination of the intracellular domain of the major Notch ligands known as Delta receptors, resulting in their endocytosis and degradation, and subsequently in the up-regulation of receptor activity and Notch pathway activation/signaling.MIB1 is highly expressed in the brain, spinal cord and ovaries, and elevated levels of both MIB1 and DAPK are found in the cytoplasm and microsomal fractions of epilepsy brain tissue.Mutation of the MIB1 gene leads to the development of Left ventricular non-compaction 7 (LVNC7), a disease caused by incomplete cardiac muscle development, characterized by a hypotrophic left ventricle (Luxánet al.2013), although it may on occasions be associated with other myocardial abnormalities (Ströllbergeret al.2002).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species Cross Reactivity
	Mouse Expected from Sequence
	Rat Expected from Sequence
	Bovine Expected from Sequence
	Dog Expected from Sequence
	N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
AntiserumPreparation	Antiserum to human MIB1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution	TRIS buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN₃)0.5% Bovine Serum Albumin
Immunogen	Synthetic peptide sequence C-DKDNTNVNADVQKLQ from the internal region of MIB1 (NP_065825.1).
Approx. Protein Concentrations	IgG concentration 0.5mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	1/16000
Immunohistology - Paraffin¹	√
Western Blotting	√	0.5	1.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹This product requires antigen retrieval using heat treatment. Sodium citrate buffer pH6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Western Blotting AHP2264 detects a band of approximately 135kDa in HEK293 lysate transiently expressing Human MIB1 tagged with myc and DYKDDDDK.
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Description	Product Code	Applications	Pack Size
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg

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Description	Product Code	Applications	Pack Size
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml
文献
1.Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.Nat Rev Mol Cell Biol. 7 (9): 678-89.

技术参数

产品应用 E ;P*; WB

产品类型 Polyclonal Antibody

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Bio-Rad MIB1抗体 AHP2264,Goat anti Human MIB1

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