Bio-Rad AHP2070 FHL1 ,Goat anti Human FHL1 antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP2070 FHL1 ,Goat anti Human FHL1 antibody

2024-10-22

Goat anti Human FHL1 antibody

货号：AHP2070

规格：0.1 mg

价格：6095

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：FHL1

物种：人

宿主：山羊

抗体亚型：IgG

荧光染料：Purified

产品介绍
Goat anti Human FHL1 antibodyrecognizes human Four and a half LIM domains protein 1, also known as FHL1, Skeletal muscle LIM-protein 1 or SLIM-1. FHL1 is a 323 amino acid ~30 kDa cytoplasmic protein containing multipleLIMdomains. FHL1 is abundantly expressed by skeletal muscle and has been linked with several muscular dystrophy-like muscle disorders, including Scapuloperoneal syndrome (Quinziiet al.2008), X-linked severe early-onset reducing body myopathy (Schlesslet al.2011), and X-linked myopathy with postural muscle atrophy (Windpassingeret al.2008).Goat anti Human FHL1 antibody is expected to recognise FHL1 isoform 5 and isoform 2, the latter being implicated as a negative regulator of RBP J- mediated transcription (Lianget al.2008).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Dog	Expected from Sequence
Mouse	√
Rat	Expected from Sequence
Bovine	Expected from Sequence
Pig	Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Antiserum Preparation	Antiserum to human FHL1 was raised by repeatedimmunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution	TRIS buffered saline.
Preservative Stabilisers	0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin
Immunogen	Synthetic peptide sequence CNKRFVFHNEQVY from the internal region of FHL1 (NP_001440.1; NP_001153171.1).
Approx. Protein Concentrations	IgG concentration 0.5 mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	1/32000
Immunohistology - Paraffin¹	√	2.0	2.5ug/ml
Western Blotting	√	0.05	0.3ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Histology Positive Control TissueHuman testis.
数据
Immunohistochemistry image of formalin fixed, paraffin embedded human testis stained using Goat anti FHL1 antibody (AHP2020) at 2.5μgml-1following heat mediated antigen retrieval in citrate buffer (pH6.0). Western blot analysis of human muscle lysate probed with Goat anti human FHL1 antibody (AHP2070).
相关试剂
Description	Product Code	Applications	Pack Size
Antigen Retrieval Buffer, pH8.0	BUF025A	IHC(P)	500 mL
文献
1. Emmanuele, V.et al.(2015) Fhl1 W122S causes loss of protein function and late-onset mild myopathy.Hum Mol Genet. 24 (3): 714-26.2. Can, T.et al.(2014) Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.Proteome Sci. 12: 25.

技术参数

产品应用 E;P*;WB

产品类型 Polyclonal Antibody

推荐用于 E;P*;WB

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Bio-Rad AHP2070 FHL1 ,Goat anti Human FHL1 antibody

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