Bio-Rad AHP206 carbonic anhydrase II,Sheep anti Human carbonic anhydrase II antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP206 carbonic anhydrase II,Sheep anti Human carbonic anhydrase II antibody

2024-10-22

Sheep anti Human carbonic anhydrase II antibody

货号：AHP206

规格：1 ml

价格：2992

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：carbonic anhydrase II

物种：人

宿主：Sheep

抗体亚型：IgG

荧光染料：Purified

产品介绍
Sheep anti Human carbonic anhydrase II antibodyrecognizes human carbonic anhydrase II, also known as Carbonate dehydratase II or Carbonic anhydrase C. Carbonic anhydrase II is a 259 amino acid ~30 kDa enzyme essential for bone resorption and osteoclast differrentiation.Mutations in the CA2 gene has been identified as the cause of osteopetrosis, autosomal recessive 3 (OPTB3), a rare disease characterized by particularly dense bone, cerebral calcification and renal tubular acidosis (Shahet al2004).Product identity is confirmed by double diffusion vs human CAII. No reactivity is seen in immunodiffusion against CAI.
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Antiserum Preparation	Antisera to human carbonic anhydrase II were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer Solution	Glycine buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN3) 0.1% EACA0.01% Benzamidine1 mM EDTA
Immunogen	Purified human carbonic anhydrase II (CAII) prepared from erythrocytes.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunodiffusion	√
Immunofluorescence	√
Western Blotting	√		1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Western BlottingSheep anti human carbonic anyhdrase II recognises a band of ~18kDa in rat adrenomedullary chromaffin cell lysates, seeSalmanet al.for details.
文献
1. Sudarov, A.et al.(2011) Ascl1 genetics reveals insights into cerebellum local circuit assembly.J Neurosci. 31: 11055-69.2. Salman, S.et al.(2013) Chronic exposure of neonatal rat adrenomedullary chromaffin cells to opioids in vitro blunts both hypoxia and hypercapnia chemosensitivity.J Physiol. 591 (Pt 2): 515-29.3. Salman, S.et al.(2014) Chronic opioids regulate KATPchannel subunit Kir6.2 and carbonic anhydrase I and II expression in rat adrenal chromaffin cells via HIF-2α and protein kinase A.Am J Physiol Cell Physiol. 307: C266-77.4. Gailly, P.et al.(2008) A novel renal carbonic anhydrase type III plays a role in proximal tubule dysfunction.Kidney Int. 74 (1): 52-61.5. Sterling, D.et al.(2002) The functional and physical relationship between the DRA bicarbonate transporter and carbonic anhydrase II.Am J Physiol Cell Physiol. 283 (5): C1522-9.6. Alvarez, L.et al.(2001) Carbonic anhydrase II associated with plasma membrane in a human pancreatic duct cell line (CAPAN-1).J Histochem Cytochem. 49 (8): 1045-53.7. Alvarez, B.V.et al.(2003) Direct extracellular interaction between carbonic anhydrase IV and the human NBC1 sodium/bicarbonate co-transporter.Biochemistry. 42 (42): 12321-9.8. Sterling, D.et al.(2002) The extracellular component of a transport metabolon. Extracellular loop 4 of the human AE1 Cl-/HCO3- exchanger binds carbonic anhydrase IV.J Biol Chem. 277 (28): 25239-46.9. Long, K.L.P.et al.(2021) Regional gray matter oligodendrocyte- and myelin-related measures are associated with differential susceptibility to stress-induced behavior in rats and humans.Transl Psychiatry. 11 (1): 631.

技术参数

产品应用 ID;IF;WB

产品类型 Polyclonal Antibody

推荐用于 ID;IF;WB

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-42534.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

Bio-Rad AHP206 carbonic anhydrase II,Sheep anti Human carbonic anhydrase II antibody

相关推荐