Bio-Rad AHP1915 M-CSF ,Rabbit anti Human M-CSF antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1915 M-CSF ,Rabbit anti Human M-CSF antibody

2024-10-22

Rabbit anti Human M-CSF antibody

货号：AHP1915

规格：0.1 mg

价格：4063

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：M-CSF

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti Human M-CSF antibodyrecognizes human M-CSF (Macrophage Colony-Stimulating Factor), a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor), and facilitates haematopoietic recovery following bone marrow transplantation.
产品详情
Target Species	Human
Product Form	Purified IgG - lyophilized
Reconstitution	Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide
Antiserum Preparation	Antiserum to human M-CSF were raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	None present
Carrier Free	Yes
Immunogen	Recombinant human M-CSF
Approx. Protein Concentrations	IgG concentration 1.0mg/ml after reconstitution
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	0.5	2.0 ug/ml
Functional Assays	√	0.02	0.03 ug/ml
Immunohistology - Paraffin¹	√
Western Blotting	√	0.1	0.2 ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Western BlottingThis antibody may be used under either reducing or non-reducing western blotting conditions withPHP220as the positive control.
数据
Formalin fixed, paraffin embedded human cervical squamous cell carcinoma stained with Rabbit anti Human M-CSF (AHP1915) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0). Formalin fixed, paraffin embedded human cervical squamous cell carcinoma stained with Rabbit anti Human M-CSF (AHP1915) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).
相关试剂
Description	Product Code	Applications	Pack Size
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB	0.5 mL
Antigen Retrieval Buffer, pH8.0	BUF025A	IHC(P)	500 mL

技术参数

产品应用 E;FN;P*;WB

产品类型 Polyclonal Antibody

推荐用于 E;FN;P*;WB

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Bio-Rad AHP1915 M-CSF ,Rabbit anti Human M-CSF antibody

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