Rabbit anti BAK (N-Terminal) antibody AHP1782 bio-rad,Rabbit anti BAK (N-Terminal) antibody

2024-10-22

Rabbit anti BAK (N-Terminal) antibody

货号:AHP1782

规格:0.1 mg

价格:5440

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:BAK (N-Terminal)

物种:其它

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti BAK (N-Terminal) antibodyrecognizes the Bcl-2 homologous antagonist/killer (Bak), an oligomeric protein localised to the outer mitochondrial membrane and the endoplasmic reticulum of healthy cells. Bak is a pro-apoptotic Bcl-2 family member similar to Bax with which it is thought to share significant homology. Mcl-1 and Bcl-xL interact with Bak and prevent the activation of Bak in healthy cells. Upon cytotoxic signalling, Mcl-1- and Bcl-xL-binding proteins (such as Noxa and Bad) displace Bak and apoptosis is induced. When activated, Bak (and Bax) permeabilises the outer membrane of the mitochondria or the ER to release proapoptogenic factors such as cytochrome c.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
Species Cross-ReactivityTarget SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Antiserum PreparationAntiserum to BAK (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.02% Sodium Azide (NaN3)
ImmunogenA peptide corresponding to 13 amino acids near the amino terminus of human BAK.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Immunohistology - Paraffin12.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg

技术参数

产品应用 wb

产品类型 Polyclonal Antibody

推荐用于 wb

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