K1461 Thermo ,EpiJET Bisulfite Conversion Kit/EpiJET Bisulfite DNA转录试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

K1461 Thermo ,EpiJET Bisulfite Conversion Kit/EpiJET Bisulfite DNA转录试剂盒

2024-10-24

EpiJET Bisulfite Conversion Kit/EpiJET Bisulfite DNA转录试剂盒

货号：K1461

规格：50 reactions

价格：923

产品类型：表观试剂

品牌：Thermo Fisher

Thermo Scientific EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged. Bisulfite converted unmethylated cytosines are detected as thymines in the following PCR.The EpiJET Bisulfite Conversion Kit integrates the thermal DNA denaturation and the bisulfite conversion reactions into one step. This step can be performed following long or short protocol, depending on the downstream application of modified DNA. The converted DNA is when bound to a membrane of a micro column for on-column desulfonation and subsequent DNA purification steps. The converted DNA is eluted in a small volume of Elution Buffer and is suitable for a number of techniques used for the methylation status analysis, including PCR, qPCR, COBRA, sequencing.

优点：

- High conversion efficiency and specificity (≥99%)- Flexibility, provided by short DNA conversion protocol- Pure DNA, suitable for downstream applications and long-term storage

数据：

图1.DNA转录流程

图2.High conversion specificity and efficiency in COBRACpG methylated plasmid DNA was converted using Thermo Scientific kit and kits of other vendors. The converted DNA was amplified using PCR and digested with restriction enzyme FastDigest AluI (#FD0014) to asses DNA conversion specificity. Due to the bisulphite conversion of DNA restriction sites for FastDigest AluI are lost leading to a different restriction pattern than the one observed in the control (amplified non-converted DNA, digested with FastDigest AluI, lane C2). The method is called Combined Bisulfite Restriction Analysis (COBRA).M - O'GeneRuler Low Range DNA Ladder, ready-to-use (#SM1203)C1 - PCR product from converted DNA templateC2 - PCR fragment from non-converted DNA template, digested with FastDigest AluI1-7 - PCR fragments from DNA converted using different bisulfite conversion kits, digested with FastDigest AluI:1 - Thermo Scientific kit, long protocol (Protocol A)2 - Thermo Scientific kit, short protocol (Protocol B)3-7 other vendors

图3.Wide range of input DNA - from 50 pg to 2 µgDifferent amounts of CpG Methylated Human Genomic DNA (#SD1131) were converted using the EpiJET Bisulfite Conversion Kit. 76 bp fragment was amplified from each converted DNA sample using Maxima SYBR Green qPCR Master Mix (2x), ROX solution provided (#K0251).

组成成分：

▪ Modification Reagent▪ Modification Solution I▪ Modification Solution II▪ Binding Buffer for Bisulfite Conversion Kit▪ Wash buffer (conc.) for Bisulfite Conversion Kit▪ Desulfonation Buffer (conc.)▪ Elution Buffer▪ DNA Purification Micro Columns & Collection Tubes

相关产品：

▪EpiJET DNA Methylation Analysis Kit (MspI/HpaII)/EpiJET DNA甲基化分析试剂盒（货号#K1441）▪EpiJET Bisulfite Conversion Kit/EpiJET Bisulfite DNA转录试剂盒（货号#K1461）▪EpiJET 5-hmC and 5-mC Analysis Kit /EpiJET 5-hmC和 5-mC分析试剂盒（货号#K1501）▪Modification Reagent/修饰试剂（货号#R2101）▪Modification Solution I/修饰试剂I（货号#R2111）▪Modification Solution II/修饰试剂II（货号#R2121）▪Wash Buffer for Bisulfite Conversion Kit (concentrated)/Bisulfite DNA转录试剂盒清晰缓冲液（浓缩）（货号#R2141）

技术参数

产品优点 1. High conversion efficiency and specificity (≥99%) 2. Flexibility, provided by short DNA conversion protocol 3. Pure DNA, suitable for downstream applications and long-term storage

产品应用 PCR qPCR Combined Bisulfite Restriction Analysis (COBRA) Sequencing

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K1461 Thermo ,EpiJET Bisulfite Conversion Kit/EpiJET Bisulfite DNA转录试剂盒

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