Bio-Rad AHP1826Z 5-Methylcytosine (5-MeC),Sheep Anti 5-Methylcytosine (5-MeC)-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP1826Z 5-Methylcytosine (5-MeC),Sheep Anti 5-Methylcytosine (5-MeC)

2024-10-22

Sheep Anti 5-Methylcytosine (5-MeC)

货号：AHP1826Z

规格：0.1 mg

价格：2465

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：5-Methylcytosine (5-MeC)

物种：Broad

宿主：Sheep

抗体亚型：IgG

荧光染料：Purified

产品介绍
Sheep Anti 5-Methylcytosine (5-MeC)recognizes the methylated form of cytosine (C), an epigenetic modification catalyzed by DNA methyltransferases resulting in the attachment of a methyl group to carbon 5. This modification alters the structure of cytosine but not its base-pairing properties. The timing and function of cytosine methylation varies amongst species with roles in such processing events as transcription and recombination. For example, in mammals, methylation occuring during early development and emrbyogensis functions in X chromosome inactivation and gene imprinting, wherease in plants, DNA methylation occurs later and affects genomic stability through silencing of transposable elements (Heet al.2011).The deamination of 5-MeC results in the formation of thymine (T) instead of uracil. Since thymine is not recognized by DNA repair enzymes, cytosine to thymine conversions can cause transition mutations. The most frequent mutations in human genetic diseases are those involving C to T transitions occurring at CG sites (Morganet al.2004). Another type of mutation involving 5-MeC involves the formation of pyrimidine dimers. Upon exposure to sunlight, pyrimidine dimers form preferentially at dipyrimidine sequences containing 5-MeC, a mutation frequently seen in human skin cancers (Pfeiferet al.2005).Sheep Anti-5-MeC is reported as suitable for use in immunocytochemistry.
产品详情
Target Species	Broad
Product Form	Purified IgG - liquid
Antiserum Preparation	Antiserum to 5-MeC was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	None present
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Immunogen	Keyhole limpet haemocyanin (KLH) conjugated 5-methylcytosine.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence	√
Immunohistology - Frozen¹	√	10ug/ml
Immunohistology - Paraffin²	√	10ug/ml
Immunoprecipitation	√	25	100ug/ml
Western Blotting	√	1:1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. ¹Using a fixation step in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining with the simultaneous detection of nuclear cytoplasmic and membrane components, as well as preservation of morphological detail.^2* Using a fixation step in cold methanol for 30 minutes followed by immersion in 0.007 M NaOH for 10-15 seconds allows staining with the simultaneous detection of nuclear cytoplasmic and membrane components, as well as preservation of morphological detail.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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文献
1. Hiura, H.et al.(2010) A tripartite paternally methylated region within the Gpr1-Zdbf2 imprinted domain on mouse chromosome 1 identified by meDIP-on-chip.Nucleic Acids Res. 38 (15): 4929-45.2. Pontes, O.et al.(2009) RNA polymerase V functions inArabidopsisinterphase heterochromatin organization independently of the 24-nt siRNA-directed DNA methylation pathway.Mol Plant. 2: 700-10.

技术参数

产品应用 IHC(F)*;IF;IP;IHC(P)*;WB

产品类型 Polyclonal Antibody

推荐用于 IHC(F)*;IF;IP;IHC(P)*;WB

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Bio-Rad AHP1826Z 5-Methylcytosine (5-MeC),Sheep Anti 5-Methylcytosine (5-MeC)

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