Rabbit anti yeast Rad9 antibody AHP1294 bio-rad,Rabbit anti yeast Rad9 antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rabbit anti yeast Rad9 antibody AHP1294 bio-rad,Rabbit anti yeast Rad9 antibody

2024-10-22

Rabbit anti yeast Rad9 antibody

货号：AHP1294

规格：50 µg

价格：4888

产品类型：免疫组化一抗

品牌：Bio-Rad

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti yeast Rad9 antibodydetectsSaccharomyces cerevisiaeRad9, a protein involved in the DNA damage checkpoint. InS. cerevisiaethis checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.Rabbit anti yeast Rad9 antibody (AHP1294) is reactive with both the phosphorylated and non-phosphorylated forms of this protein.
产品详情
Target Species	Yeast
Product Form	Purified IgG - liquid
Antiserum Preparation	Antisera to yeast Rad9 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)
Immunogen	Synthetic peptide corresponding to aa 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin (KLH).
Approx. Protein Concentrations	IgG concentration 0.5mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	1/2000	1/4000
Immunohistology - Frozen	√	1/50	1/250
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
Description	Product Code	Applications	Pack Size
Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E,WB	1mg
Goat anti Rabbit IgG (H/L):HRP	STAR124P	C,E,WB	1mg
文献
1.Toh, G.W. & Lowndes, N.F. (2003) Role of the Saccharomyces cerevisiae Rad9 protein in sensing and responding to DNA damage.Biochem Soc Trans. 31 (Pt 1): 242-6.2.Sweeney, F.D.et al.(2005) Saccharomyces cerevisiae Rad9 acts as a Mec1 adaptor to allow Rad53 activation.Curr Biol. 15 (15): 1364-75.

技术参数

产品应用 elisa；wb

产品类型 Polyclonal Antibody

推荐用于 elisa；wb

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Rabbit anti yeast Rad9 antibody AHP1294 bio-rad,Rabbit anti yeast Rad9 antibody

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