Rabbit anti Human WNT-1 antibody AHP1287 bio-rad,Rabbit anti Human WNT-1 antibody

2024-10-22

Rabbit anti Human WNT-1 antibody

货号:AHP1287

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human WNT-1 antibodyrecognizes Wnt-1, formerly known as int-1, a 343 amino acid non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer.Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumor-like virus (Wainwright 1988). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression.
产品详情
Target SpeciesHuman
Product FormPurified IgG - lyophilized
ReconstitutionReconstitute with 0.1ml distilled water.For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum PreparationAntisera to human Wnt-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer SolutionPhosphate buffered saline
Preservative StabilisersNone present
ImmunogenRecombinant human Wnt-1.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml after reconstitution
RegulatoryFor research purposes only
Guarantee6 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.52.0ug/ml
Functional Assays0.10.2ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Brown, A.M.et al.(1987) Identification of protein products encoded by the proto-oncogene int-1.Mol Cell Biol. 7 (11): 3971-7.

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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