Bio-Rad AHP1456 GLI3,Rabbit anti Human GLI3 antibody

2024-10-22

Rabbit anti Human GLI3 antibody

货号:AHP1456

规格:50 µg

价格:6095

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:GLI3

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human GLI3 antibodyrecognizes the Human Zinc Finger protein 3 (GLI-3), also known as GLI-Kruppel family member 3, a 169.8 kDa protein belonging to the GLI C2H2-type zinc finger protein of transcription factors.GLI-3 and the related GLI-2 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI-2 being primarily a transcriptional activator while GLI-3 is a transcriptional repressor. GLI-3 plays a role in the development of the limbs and brain. It is expressed in a wide range of adult tissues including the colon, lung, spleen, testis and placenta.Defects in GLI3 are causative of a range of disease states including Pallister-Hall syndrome, type IV preaxial polydactyly, acrocallosal syndrome (ACS) and Greig cephalo-poly-syndactyly syndrome. GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell.
产品详情

Target Species

Human

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human GLI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN3)

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Immunogen

Synthetic peptide corresponding to amino acid sequence 41-57 of human Gli3 protein.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/6000

Immunohistology - Paraffin1

1.25ug/ml

Western Blotting

1/500
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Histology Positive Control Tissue
  • Human brain, glioblastoma
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数据

Staining of formalin fixed, paraffin embedded human glioblastoma with Rabbit anti Human GLI3 (AHP1456)

技术参数

产品应用 ELISA;IHC(P)*;WB

产品类型 Polyclonal Antibody

推荐用于 ELISA;IHC(P)*;WB

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